ArticleProceedings of the National Academy of Sciences of the United States of America2002
Tumor attenuation by combined heparan sulfate and polyamine depletion.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 119 citations in OpenAlex.
- Article
- KLF4 is required for suppression of histamine synthesis by polyamines during bone marrow-derived mast cell differentiation.PloS one · 2020Article
- The Expression, Regulation, and Biomarker Potential of Glypican-1 in Cancer.Frontiers in oncology · 2019Review
- Facile saccharide-free mimetics that recapitulate key features of glycosaminoglycan sulfation patterns.Chemical science · 2018Article
- Cancer Cell Glycocalyx and Its Significance in Cancer Progression.International journal of molecular sciences · 2018Review
- Tumor antigen glycosaminoglycan modification regulates antibody-drug conjugate delivery and cytotoxicity.Oncotarget · 2017Article
- Novel Heparin Receptor Transmembrane Protein 184a Regulates Angiogenesis in the Adult Zebrafish Caudal Fin.Frontiers in physiology · 2017Article
- Heparan sulfate proteoglycans mediate renal carcinoma metastasis.International journal of cancer · 2016Article
- Pingyangmycin and Bleomycin Share the Same Cytotoxicity Pathway.Molecules (Basel, Switzerland) · 2016Article
- Analysis of nucleotide diphosphate sugar dehydrogenases reveals family and group-specific relationships.FEBS open bio · 2016Article
- Probing the acceptor active site organization of the human recombinant β1,4-galactosyltransferase 7 and design of xyloside-based inhibitors.The Journal of biological chemistry · 2015Article
- Heparan sulfate containing unsubstituted glucosamine residues: biosynthesis and heparanase-inhibitory activity.The Journal of biological chemistry · 2014Article
- Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for their internalization and functional activity.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Human UDP-α-D-xylose synthase and Escherichia coli ArnA conserve a conformational shunt that controls whether xylose or 4-keto-xylose is produced.Biochemistry · 2012Article
- Transition state analogue inhibitors of human methylthioadenosine phosphorylase and bacterial methylthioadenosine/S-adenosylhomocysteine nucleosidase incorporating acyclic ribooxacarbenium ion mimics.Bioorganic & medicinal chemistry · 2012Article
- DFMO: targeted risk reduction therapy for colorectal neoplasia.Best practice & research. Clinical gastroenterology · 2011Review
- Small lytic peptides escape the inhibitory effect of heparan sulfate on the surface of cancer cells.BMC cancer · 2011Article
- Determinants of the differential antizyme-binding affinity of ornithine decarboxylase.PloS one · 2011Article
- Current status of the polyamine research field.Methods in molecular biology (Clifton, N.J.) · 2011Review
- Polyamines are present in mast cell secretory granules and are important for granule homeostasis.PloS one · 2010Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Cells depend on polyamines for growth and their depletion represents a strategy for the treatment of cancer. Polyamines assemble de novo through a pathway sensitive to the inhibitor, alpha-difluoromethylornithine (DFMO). However, the presence of cell-surface heparan sulfate proteoglycans may provide a salvage pathway for uptake of circulating polyamines, thereby sparing cells from the cytostatic effect of DFMO. Here we show that genetic or pharmacologic manipulation of proteoglycan synthesis in the presence of DFMO inhibits cell proliferation in vitro and in vivo. In cell culture, mutant cells lacking heparan sulfate were more sensitive to the growth inhibitory effects of DFMO than wild-type cells or mutant cells transfected with the cDNA for the missing biosynthetic enzyme. Moreover, extracellular polyamines did not restore growth of mutant cells, but completely reversed the inhibitory effect of DFMO in wild-type cells. In a mouse model of experimental metastasis, DFMO provided in the water supply also dramatically diminished seeding and growth of tumor foci in the lungs by heparan sulfate-deficient mutant cells compared with the controls. Wild-type cells also formed tumors less efficiently in mice fed both DFMO and a xylose-based inhibitor of heparan sulfate proteoglycan assembly. The effect seemed to be specific for heparan sulfate, because a different xyloside known to affect only chondroitin sulfate did not inhibit tumor growth. Hence, combined inhibition of heparan sulfate assembly and polyamine synthesis may represent an additional strategy for cancer therapy.
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