ArticleNucleic acids research2002
The DT40 web site: sampling and connecting the genes of a B cell line.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed.
- MicroRNA expression profiles in avian haemopoietic cells.Frontiers in genetics · 2013Article
- Chromatin structure regulates gene conversion.PLoS biology · 2007Article
- Identification and functional characterization of chicken toll-like receptor 5 reveals a fundamental role in the biology of infection with Salmonella enterica serovar typhimurium.Infection and immunity · 2005Article
- Identification and characterization of a functional, alternatively spliced Toll-like receptor 7 (TLR7) and genomic disruption of TLR8 in chickens.Immunology · 2005Article
- Development of a cDNA array for chicken gene expression analysis.BMC genomics · 2005Article
- Evaluation of the chicken transcriptome by SAGE of B cells and the DT40 cell line.BMC genomics · 2004Article
- Insight into lymphoid development by gene expression profiling of avian B cells.Immunogenetics · 2003Article
- Conditional human VEGF-mediated vascularization in chicken embryos using a novel temperature-inducible gene regulation (TIGR) system.Nucleic acids research · 2003Article
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7 authors.
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Abstract
Thousands of new vertebrate genes have been discovered and genetic systems are needed to address their functions at the cellular level. The chicken B cell line DT40 allows efficient gene disruptions due to its high homologous recombination activity. However, cloning the gene of interest is often cumbersome, since relatively few chicken cDNA sequences are present in the public databases. In addition, the accumulation of multiple mutations within the same cell clone is limited by the consumption of one drug-resistance marker for each transfection. Here, we present the DT40 web site (http://genetics.hpi.uni-hamburg.de/dt40.html), which includes a comprehensive database of chicken bursal ESTs to identify disruption candidate genes and recyclable marker cassettes based on the loxP system. These freely available resources greatly facilitate the analysis of genes and genetic networks.
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