ArticleJournal of bacteriology2002
Phylogeny and functional expression of ribulose 1,5-bisphosphate carboxylase/oxygenase from the autotrophic ammonia-oxidizing bacterium Nitrosospira sp. isolate 40KI.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 40 citations in OpenAlex.
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- Carbon content, carbon fixation yield and dissolved organic carbon release from diverse marine nitrifiers.Limnology and oceanography · 2023Article
- Comparative Proteomics of Three Species of Ammonia-Oxidizing Bacteria.Frontiers in microbiology · 2018Article
- Complete genome of Nitrosospira briensis C-128, an ammonia-oxidizing bacterium from agricultural soil.Standards in genomic sciences · 2016Article
- The complete genome sequences of sulfur-oxidizing Gammaproteobacteria Sulfurifustis variabilis skN76(T) and Sulfuricaulis limicola HA5(T).Standards in genomic sciences · 2016Article
- Making a living while starving in the dark: metagenomic insights into the energy dynamics of a carbonate cave.The ISME journal · 2014Article
- Ammonia oxidation coupled to CO2 fixation by archaea and bacteria in an agricultural soil.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Complete genome sequence of Nitrosospira multiformis, an ammonia-oxidizing bacterium from the soil environment.Applied and environmental microbiology · 2008Article
- Diversity and structure of bacterial chemolithotrophic communities in pine forest and agroecosystem soils.Applied and environmental microbiology · 2005Article
- Analysis of facultative lithotroph distribution and diversity on volcanic deposits by use of the large subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase.Applied and environmental microbiology · 2004Article
- Integron diversity in heavy-metal-contaminated mine tailings and inferences about integron evolution.Applied and environmental microbiology · 2004Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The autotrophic ammonia-oxidizing bacteria (AOB), which play an important role in the global nitrogen cycle, assimilate CO(2) by using ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO). Here we describe the first detailed study of RubisCO (cbb) genes and proteins from the AOB. The cbbLS genes from Nitrosospira sp. isolate 40KI were cloned and sequenced. Partial sequences of the RubisCO large subunit (CbbL) from 13 other AOB belonging to the beta and gamma subgroups of the class Proteobacteria are also presented. All except one of the beta-subgroup AOB possessed a red-like type I RubisCO with high sequence similarity to the Ralstonia eutropha enzyme. All of these new red-like RubisCOs had a unique six-amino-acid insert in CbbL. Two of the AOB, Nitrosococcus halophilus Nc4 and Nitrosomonas europaea Nm50, had a green-like RubisCO. With one exception, the phylogeny of the AOB CbbL was very similar to that of the 16S rRNA gene. The presence of a green-like RubisCO in N. europaea was surprising, as all of the other beta-subgroup AOB had red-like RubisCOs. The green-like enzyme of N. europaea Nm50 was probably acquired by horizontal gene transfer. Functional expression of Nitrosospira sp. isolate 40KI RubisCO in the chemoautotrophic host R. eutropha was demonstrated. Use of an expression vector harboring the R. eutropha cbb control region allowed regulated expression of Nitrosospira sp. isolate 40KI RubisCO in an R. eutropha cbb deletion strain. The Nitrosospira RubisCO supported autotrophic growth of R. eutropha with a doubling time of 4.6 h. This expression system may allow further functional analysis of AOB cbb genes.
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