ArticleJournal of bacteriology2002
Inactivation of the spirochete recA gene results in a mutant with low viability and irregular nucleoid morphology.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Genome-Editing Tools for Lactic Acid Bacteria: Past Achievements, Current Platforms, and Future Directions.International journal of molecular sciences · 2025Review
- The VapBC-4 Characterization Indicates It Is a Bona Fide Toxin-Antitoxin Module ofMicroorganisms · 2025Article
- Creating a Library of Random Transposon Mutants in Leptospira.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Screening of a Leptospira biflexa mutant library to identify genes involved in ethidium bromide tolerance.Applied and environmental microbiology · 2014Article
- Leptospira interrogans serovar copenhageni harbors two lexA genes involved in SOS response.PloS one · 2013Article
- Positive regulation of Leptospira interrogans kdp expression by KdpE as Demonstrated with a novel β-galactosidase reporter in Leptospira biflexa.Applied and environmental microbiology · 2012Article
- Three-dimensional structures of pathogenic and saprophytic Leptospira species revealed by cryo-electron tomography.Journal of bacteriology · 2012Article
- Frequent mutations within the genomic magnetosome island of Magnetospirillum gryphiswaldense are mediated by RecA.Journal of bacteriology · 2011Article
- Leptospira as an emerging pathogen: a review of its biology, pathogenesis and host immune responses.Future microbiology · 2010Review
- Targeted mutagenesis in pathogenic Leptospira species: disruption of the LigB gene does not affect virulence in animal models of leptospirosis.Infection and immunity · 2008Article
- Borrelia burgdorferi vlsE antigenic variation is not mediated by RecA.Infection and immunity · 2008Article
- Comparative and functional genomic analyses of iron transport and regulation in Leptospira spp.Journal of bacteriology · 2006Article
- Borrelia burgdorferi ftsZ plays a role in cell division.Journal of bacteriology · 2006Article
- A homozygous recA mutant of Synechocystis PCC6803: construction strategy and characteristics eliciting a novel RecA independent UVC resistance in dark.Molecular genetics and genomics : MGG · 2005Article
- Isolation and characterization of FecA- and FeoB-mediated iron acquisition systems of the spirochete Leptospira biflexa by random insertional mutagenesis.Journal of bacteriology · 2005Article
- Random insertional mutagenesis of Leptospira interrogans, the agent of leptospirosis, using a mariner transposon.Journal of bacteriology · 2005Article
- Functional properties of Borrelia burgdorferi recA.Journal of bacteriology · 2004Article
- Transgenic expression of RecA of the spirochetes Borrelia burgdorferi and Borrelia hermsii in Escherichia coli revealed differences in DNA repair and recombination phenotypes.Journal of bacteriology · 2004Article
- Temperature-dependent hypermutational phenotype in recA mutants of Thermus thermophilus HB27.Journal of bacteriology · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, we have shown the first evidence for allelic exchange in Leptospira spp. By using the same methodology, the cloned recA of Leptospira biflexa was interrupted by a kanamycin resistance cassette, and the mutated allele was then introduced into the L. biflexa chromosome by homologous recombination. The recA double-crossover mutant showed poor growth in liquid media and was considerably more sensitive to DNA-damaging agents such as mitomycin C and UV light than the wild-type strain. The efficiency of plating of the recA mutant was about 10% of that of the parent strain. In addition, microscopic observation of the L. biflexa recA mutant showed cells that are more elongated than those of the wild-type strain. Fluorescent microscopy of stained cells of the L. biflexa wild-type strain revealed that chromosomal DNA is distributed throughout most of the length of the cell. In contrast, the recA mutant showed aberrant nucleoid morphologies, i.e., DNA is condensed at the midcell. Our data indicate that L. biflexa RecA plays a major role in ensuring cell viability via mechanisms such as DNA repair and, indirectly, active chromosome partitioning.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.