ArticleJournal of bacteriology2002
Diversity of Tn4001 transposition products: the flanking IS256 elements can form tandem dimers and IS circles.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- The novel pseudo-compound transposon TnMicrobial genomics · 2026Article
- Article
- Targeted IS-element sequencing uncovers transposition dynamics during selective pressure in enterococci.PLoS pathogens · 2023Article
- Species Delineation and Comparative Genomics within theJournal of clinical microbiology · 2023Review
- Insertion sequences and other mobile elements associated with antibiotic resistance genes inMicrobial genomics · 2022Article
- How Do Transposable Elements Activate Expression of Transcriptionally Silent Antibiotic Resistance Genes?International journal of molecular sciences · 2022Review
- The dynamic network of IS30 transposition pathways.PloS one · 2022Article
- The IS6 family, a clinically important group of insertion sequences including IS26.Mobile DNA · 2021Review
- Structures of ISCth4 transpososomes reveal the role of asymmetry in copy-out/paste-in DNA transposition.The EMBO journal · 2021Article
- Detection of mobile genetic elements associated with antibiotic resistance in Salmonella enterica using a newly developed web tool: MobileElementFinder.The Journal of antimicrobial chemotherapy · 2021Article
- Tackling Multidrug Resistance in Streptococci - From Novel Biotherapeutic Strategies to Nanomedicines.Frontiers in microbiology · 2020Review
- Staphylococcal Plasmids, Transposable and Integrative Elements.Microbiology spectrum · 2018Review
- Double insertion of transposable elements provides a substrate for the evolution of satellite DNA.Genome research · 2018Article
- Genetic Basis of Emerging Vancomycin, Linezolid, and Daptomycin Heteroresistance in a Case of Persistent Enterococcus faecium Bacteremia.Antimicrobial agents and chemotherapy · 2018Article
- The physiological opportunism of Desulfitobacterium hafniense strain TCE1 towards organohalide respiration with tetrachloroethene.Applied and environmental microbiology · 2012Article
- Protein-DNA interactions define the mechanistic aspects of circle formation and insertion reactions in IS2 transposition.Mobile DNA · 2012Article
- Soluble expression, purification and characterization of the full length IS2 Transposase.Mobile DNA · 2011Article
- Characterization of the transposase encoded by IS256, the prototype of a major family of bacterial insertion sequence elements.Journal of bacteriology · 2010Article
- A model for the molecular organisation of the IS911 transpososome.Mobile DNA · 2010Article
- Integrating prokaryotes and eukaryotes: DNA transposases in light of structure.Critical reviews in biochemistry and molecular biology · 2010Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We show that both flanking IS256 elements carried by transposon Tn4001 are capable of generating head-to-tail tandem copies and free circular forms, implying that both are active. Our results suggest that the tandem structures arise from dimeric copies of the donor or vector plasmid present in the population by a mechanism in which an IS256 belonging to one Tn4001 copy attacks an IS256 end carried by the second Tn4001 copy. The resulting structures carry abutted left (inverted left repeat [IRL]) and right (inverted right repeat [IRR]) IS256 ends. Examination of the junction sequence suggested that it may form a relatively good promoter capable of driving transposase synthesis in Escherichia coli. This behavior resembles that of an increasing number of bacterial insertion sequences which generate integrative junctions as part of the transposition cycle. Sequence analysis of the IRL-IRR junctions demonstrated that attack of one end by the other is largely oriented (IRL attacks IRR). Our experiments also defined the functional tips of IS256 as the tips predicted from sequence alignments, confirming that the terminal 4 bp at each end are indeed different. The appearance of these multiple plasmid and transposon forms indicates that care should be exercised when Tn4001 is used in transposition mutagenesis. This is especially true when it is used with naturally transformable hosts, such as Streptococcus pneumoniae, in which reconstitution of the donor plasmid may select for higher-order multimers.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.