ArticleBiophysical journal2002
Relationship of lipid rafts to transient confinement zones detected by single particle tracking.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 169 papers.
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Who cites it
169 citing papers in PubMed.
- Effect of fish meal supplementation on spatial distribution of lipid microdomains and on the lateral mobility of membrane-bound prostaglandin FDomestic animal endocrinology · 2017Trial
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- Volumetric Single-Molecule Tracking Inside Subcellular Structures.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- High-Speed Interferometric Scattering Tracking Microscopy of Compartmentalized Lipid Diffusion in Living Cells.Chemphyschem : a European journal of chemical physics and physical chemistry · 2025Article
- Modeling Diffusion Between Regions with Different Diffusion Coefficients.IEEE transactions on molecular, biological, and multi-scale communications · 2024Article
- Membrane staining and phospholipid tracking in Pseudomonas aeruginosa PAO1 using the phosphatidylcholine mimic propargyl-choline.Access microbiology · 2024Article
- The LDL receptor is regulated by membrane cholesterol as revealed by fluorescence fluctuation analysis.Biophysical journal · 2023Article
- Development of ultrafast camera-based single fluorescent-molecule imaging for cell biology.The Journal of cell biology · 2023Article
- Glycosphingolipid and Glycosylphosphatidylinositol Affect Each Other in and on the Cell.Chembiochem : a European journal of chemical biology · 2023Review
- An agent-based approach for modelling and simulation of glycoprotein VI receptor diffusion, localisation and dimerisation in platelet lipid rafts.Scientific reports · 2023Article
- Modulation of a rapid neurotransmitter receptor-ion channel by membrane lipids.Frontiers in cell and developmental biology · 2023Review
- Recombination betweenAnalytical chemistry · 2022Article
- Precise Detection and Visualization of Nanoscale Temporal Confinement in Single-Molecule Tracking Analysis.Membranes · 2022Article
- Correlative nanophotonic approaches to enlighten the nanoscale dynamics of living cell membranes.Biochemical Society transactions · 2021Review
- Intertwined and Finely Balanced: Endoplasmic Reticulum Morphology, Dynamics, Function, and Diseases.Cells · 2021Review
- Detecting Transient Trapping from a Single Trajectory: A Structural Approach.Entropy (Basel, Switzerland) · 2021Article
- Determination of the size of lipid rafts studied through single-molecule FRET simulations.Biophysical journal · 2021Article
- A method for imaging single molecules at the plasma membrane of live cells within tissue slices.The Journal of general physiology · 2021Article
- TRAIT2D: a Software for Quantitative Analysis of Single Particle Diffusion Data.F1000Research · 2021Article
- Real-time observation of dynamic structure of liquid-vapor interface at nanometer resolution in electron irradiated sodium chloride crystals.Scientific reports · 2020Article
109 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
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Abstract
We examined the physical and chemical characteristics of transient confinement zones (TCZs) that are detected in single particle trajectories of molecules moving within the membrane of C3H 10T1/2 murine fibroblasts and their relationship to "rafts." We studied the lateral movement of different membrane molecules thought to partition to varying degrees into or out of the putative lipid domains known as rafts. We found that lipid analogs spend significantly less time in TCZs compared with Thy-1, a glycosylphosphatidylinositol-anchored protein, and GM1, a glycosphingolipid. For Thy-1, we found that zone abundance was markedly reduced by cholesterol extraction, suggesting that a major source of the observed temporary confinement is related to the presence of raft domains. More detailed analysis of particle trajectories reveals that zones can be revisited even tens of seconds after the original escape and that diffusion within the zones is reduced by a factor of approximately 2, consistent with the zone being a cholesterol-rich liquid-ordered phase. Surprisingly, transient confinement was not strongly temperature dependent. Overall, our data demonstrate that there are raft-related domains present in certain regions of the plasma membrane of C3H cells, which can persist for tens of seconds.
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