ArticleThe Journal of experimental medicine2001
Shear-dependent eosinophil transmigration on interleukin 4-stimulated endothelial cells: a role for endothelium-associated eotaxin-3.
Article in The Journal of experimental medicine, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 99 citations in OpenAlex.
- Review
- CCR3-dependent eosinophil recruitment is regulated by sialyltransferase ST3Gal-IV.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- The Role of Heparan Sulfate in CCL26-Induced Eosinophil Chemotaxis.International journal of molecular sciences · 2022Article
- An endothelial microRNA-1-regulated network controls eosinophil trafficking in asthma and chronic rhinosinusitis.The Journal of allergy and clinical immunology · 2020Article
- Dynamic transcriptomic analysis of Ischemic Injury in a Porcine Pre-Clinical Model mimicking Donors Deceased after Circulatory Death.Scientific reports · 2018Article
- Abnormal cannabidiol attenuates experimental colitis in mice, promotes wound healing and inhibits neutrophil recruitment.Journal of inflammation (London, England) · 2016Article
- The chemokine system in innate immunity.Cold Spring Harbor perspectives in biology · 2015Review
- IL-4 attenuates Th1-associated chemokine expression and Th1 trafficking to inflamed tissues and limits pathogen clearance.PloS one · 2013Article
- Inflammatory cell trafficking across the blood-brain barrier: chemokine regulation and in vitro models.Immunological reviews · 2012Review
- CXCL12-induced monocyte-endothelial interactions promote lymphocyte transmigration across an in vitro blood-brain barrier.Science translational medicine · 2012Article
- Chemokines and their receptors in the allergic airway inflammatory process.Clinical reviews in allergy & immunology · 2011Review
- Regulation of eotaxin-3/CCL26 expression in human monocytic cells.Immunology · 2010Article
- Neutralizing endogenous chemokines with small molecules. Principles and potential therapeutic applications.Pharmacology & therapeutics · 2010Review
- Intravascular immunity: the host-pathogen encounter in blood vessels.Nature reviews. Immunology · 2009Review
- Lymphocyte crawling and transendothelial migration require chemokine triggering of high-affinity LFA-1 integrin.Immunity · 2009Article
- Leukocyte transmigration requires kinesin-mediated microtubule-dependent membrane trafficking from the lateral border recycling compartment.The Journal of experimental medicine · 2008Article
- Recoil and stiffening by adherent leukocytes in response to fluid shear.Biophysical journal · 2008Article
- Assays of transendothelial migration in vitro.Methods in enzymology · 2008Article
- Chemokines and their receptors as potential targets for the treatment of asthma.British journal of pharmacology · 2007Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leukocyte infiltration into inflammatory sites is regulated by the expression of adhesion and activation proteins, yet the role of these proteins in shear-dependent transmigration is poorly understood. We examined eosinophil recruitment on cytokine-stimulated human umbilical vein endothelial cells (HUVECs) under laminar flow conditions. Eosinophils rapidly transmigrated on interleukin (IL)-4-, but not TNF-stimulated HUVECs. Transmigration was shear dependent, with up to 90% of eosinophils transmigrating in the presence of shear and less than 25% of cells transmigrating under static conditions. Eosinophils express CC chemokine receptor CCR3 and are responsive to various CC chemokines. The effects of chemokines are mediated primarily through G(alpha)i, which is pertussis toxin sensitive. Greater than 65% of shear-dependent eosinophil transmigration on IL-4-stimulated HUVECs was blocked by either pertussis toxin or by an anti-CCR3 monoclonal antibody. Using reverse transcription polymerase chain reaction (RT-PCR) and Western blots, we found that IL-4-stimulated HUVECs produce both mRNA and protein for eotaxin-3. Eotaxin-3 was both released by HUVECs and expressed on the endothelial cell surface. Pretreatment of HUVECs with an anti-eotaxin-3 antibody blocked eosinophil transmigration to the same extent as an anti-CCR3 antibody. These results indicate that IL-4-stimulated HUVECs support shear-dependent eosinophil transmigration by upregulating eotaxin-3, and that surface association is critical for the role of eotaxin-3 in transmigration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.