ArticleThe Journal of clinical investigation2001
Cellular bicarbonate protects rat duodenal mucosa from acid-induced injury.
Article in The Journal of clinical investigation, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
- TolCV1 inhibition by NPPB rendersAntimicrobial agents and chemotherapy · 2025Article
- Enteric Tuft Cells in Host-Parasite Interactions.Pathogens (Basel, Switzerland) · 2021Review
- The Role of pHFrontiers in cell and developmental biology · 2021Review
- Duodenal chemosensory system: enterocytes, enteroendocrine cells, and tuft cells.Current opinion in gastroenterology · 2020Review
- Wireless telemetry and cystic fibrosis: just the pHacts.Digestive diseases and sciences · 2013Article
- Essential role of the electroneutral Na+-HCO3- cotransporter NBCn1 in murine duodenal acid-base balance and colonic mucus layer build-up in vivo.The Journal of physiology · 2013Article
- Gastro protective properties of the novel prostone SPI-8811 against acid-injured porcine mucosa.World journal of gastroenterology · 2012Article
- The electroneutral Na⁺:HCO₃⁻ cotransporter NBCn1 is a major pHi regulator in murine duodenum.The Journal of physiology · 2012Article
- Role for ion transport in porcine vocal fold epithelial defense to acid challenge.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2012Article
- Na+/HCO3- cotransporter immunoreactivity changes in neurons and expresses in astrocytes in the gerbil hippocampal CA1 region after ischemia/reperfusion.Neurochemical research · 2011Article
- Luminal chemosensing in the duodenal mucosa.Acta physiologica (Oxford, England) · 2011Review
- Duodenal chemosensing and mucosal defenses.Digestion · 2011Article
- Caveolin-1-dependent occludin endocytosis is required for TNF-induced tight junction regulation in vivo.The Journal of cell biology · 2010Article
- Luminal L-glutamate enhances duodenal mucosal defense mechanisms via multiple glutamate receptors in rats.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- Luminal chemosensing and upper gastrointestinal mucosal defenses.The American journal of clinical nutrition · 2009Article
- Duodenal acidity "sensing" but not epithelial HCO3- supply is critically dependent on carbonic anhydrase II expression.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Intestinal alkaline phosphatase regulates protective surface microclimate pH in rat duodenum.The Journal of physiology · 2009Article
- Epithelial carbonic anhydrases facilitate PCO2 and pH regulation in rat duodenal mucosa.The Journal of physiology · 2006Article
- Choosing sides in the battle against gastric acid.The Journal of clinical investigation · 2001Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
Secretion of bicarbonate from epithelial cells is considered to be the primary mechanism by which the duodenal mucosa is protected from acid-related injury. Against this view is the finding that patients with cystic fibrosis, who have impaired duodenal bicarbonate secretion, are paradoxically protected from developing duodenal ulcers. Therefore, we hypothesized that epithelial cell intracellular pH regulation, rather than secreted extracellular bicarbonate, was the principal means by which duodenal epithelial cells are protected from acidification and injury. Using a novel in vivo microscopic method, we have measured bicarbonate secretion and epithelial cell intracellular pH (pH(i)), and we have followed cell injury in the presence of the anion transport inhibitor DIDS and the Cl(-) channel inhibitor, 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB). DIDS and NPPB abolished the increase of duodenal bicarbonate secretion following luminal acid perfusion. DIDS decreased basal pH(i), whereas NPPB increased pH(i); DIDS further decreased pH(i) during acid challenge and abolished the pH(i) overshoot over baseline observed after acid challenge, whereas NPPB attenuated the fall of pH(i) and exaggerated the overshoot. Finally, acid-induced epithelial injury was enhanced by DIDS and decreased by NPPB. The results support the role of intracellular bicarbonate in the protection of duodenal epithelial cells from luminal gastric acid.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.