ArticleThe Journal of cell biology2001
Sorting of Golgi resident proteins into different subpopulations of COPI vesicles: a role for ArfGAP1.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
77 citing papers in PubMed, 183 citations in OpenAlex.
- ARF: the most misunderstood GTPase I ever knew - why study ARF GAPs.Frontiers in molecular biosciences · 2025Review
- Arf GTPase-activating proteins SMAP1 and AGFG2 regulate the size of Weibel-Palade bodies and exocytosis of von Willebrand factor.Biology open · 2021Article
- The Golgi complex: a hub of the secretory pathway.BMB reports · 2021Review
- Article
- Genome-Wide Identification of Genes Involved in General Acid Stress and Fluoride Toxicity inFrontiers in microbiology · 2020Article
- Visualization of secretory cargo transport within the Golgi apparatus.The Journal of cell biology · 2019Article
- GORAB scaffolds COPI at the trans-Golgi for efficient enzyme recycling and correct protein glycosylation.Nature communications · 2019Article
- A Kinetic View of Membrane Traffic Pathways Can Transcend the Classical View of Golgi Compartments.Frontiers in cell and developmental biology · 2019Review
- Article
- The Golgin protein Coy1 functions in intra-Golgi retrograde transport and interacts with the COG complex and Golgi SNAREs.Molecular biology of the cell · 2017Article
- Study of Ethanol-Induced Golgi Disorganization Reveals the Potential Mechanism of Alcohol-Impaired N-Glycosylation.Alcoholism, clinical and experimental research · 2016Article
- p24 family proteins: key players in the regulation of trafficking along the secretory pathway.Protoplasma · 2016Review
- The ArfGAP2/3 Glo3 and ergosterol collaborate in transport of a subset of cargoes.Biology open · 2015Article
- The Arf6 GTPase-activating proteins ARAP2 and ACAP1 define distinct endosomal compartments that regulate integrin α5β1 traffic.The Journal of biological chemistry · 2014Article
- Golgi compartmentation and identity.Current opinion in cell biology · 2014Review
- ArfGAPs: key regulators for receptor sorting.Receptors & clinical investigation · 2014Article
- Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae.eLife · 2014Article
- Article
- Phospholipase D2 is involved in the formation of Golgi tubules and ArfGAP1 recruitment.PloS one · 2014Article
- Oxysterol-binding protein (OSBP) is required for the perinuclear localization of intra-Golgi v-SNAREs.Molecular biology of the cell · 2013Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present evidence for two subpopulations of coatomer protein I vesicles, both containing high amounts of Golgi resident proteins but only minor amounts of anterograde cargo. Early Golgi proteins p24alpha2, beta1, delta1, and gamma3 are shown to be sorted together into vesicles that are distinct from those containing mannosidase II, a glycosidase of the medial Golgi stack, and GS28, a SNARE protein of the Golgi stack. Sorting into each vesicle population is Arf-1 and GTP hydrolysis dependent and is inhibited by aluminum and beryllium fluoride. Using synthetic peptides, we find that the cytoplasmic domain of p24beta1 can bind Arf GTPase-activating protein (GAP)1 and cause direct inhibition of ArfGAP1-mediated GTP hydrolysis on Arf-1 bound to liposomes and Golgi membranes. We propose a two-stage reaction to explain how GTP hydrolysis constitutes a prerequisite for sorting of resident proteins, yet becomes inhibited in their presence.
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Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.