ArticleInfection and immunity2002
Genomic approach for analysis of surface proteins in Chlamydia pneumoniae.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 200 citations in OpenAlex.
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- Identification of Subunits for Novel Universal Vaccines against Three Predominant Serogroups and the Emerging O145 among Avian Pathogenic Escherichia coli by Pan-RV Pipeline.Applied and environmental microbiology · 2023Article
- Recent Advances in Genomics-Based Approaches for the Development of Intracellular Bacterial Pathogen Vaccines.Pharmaceutics · 2022Review
- Combing Immunoinformatics with Pangenome Analysis To Design a Multiepitope Subunit Vaccine against Klebsiella pneumoniae K1, K2, K47, and K64.Microbiology spectrum · 2022Article
- Vaccines and Immunoinformatics for Vaccine Design.Advances in experimental medicine and biology · 2022Article
- Genomic Analysis ofMicroorganisms · 2021Article
- A primary Chlamydia trachomatis genital infection of rhesus macaques identifies new immunodominant B-cell antigens.PloS one · 2021Article
- Insights Into aFrontiers in cellular and infection microbiology · 2020Article
- PanRV: Pangenome-reverse vaccinology approach for identifications of potential vaccine candidates in microbial pangenome.BMC bioinformatics · 2019Article
- BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design againstAntibodies (Basel, Switzerland) · 2018Article
- In silico prediction of T- and B-cell epitopes in PmpD: First step towards to the design of a Chlamydia trachomatis vaccine.Biomedical journal · 2018Article
- Comparative proteomic analysis of Xanthomonas citri ssp. citri periplasmic proteins reveals changes in cellular envelope metabolism during in vitro pathogenicity induction.Molecular plant pathology · 2018Article
- A Gene-Based Positive Selection Detection Approach to Identify Vaccine Candidates UsingFrontiers in genetics · 2018Article
- Proteomics, Bioinformatics and Structure-Function Antigen Mining For Gonorrhea Vaccines.Frontiers in immunology · 2018Review
- Immunoprotective potential of BamA, the outer membrane protein assembly factor, against MDR Acinetobacter baumannii.Scientific reports · 2017Article
- Towards developing a vaccine for rheumatic heart disease.Global cardiology science & practice · 2017Review
- VacSol: a high throughput in silico pipeline to predict potential therapeutic targets in prokaryotic pathogens using subtractive reverse vaccinology.BMC bioinformatics · 2017Article
- The Chlamydia pneumoniae Adhesin Pmp21 Forms Oligomers with Adhesive Properties.The Journal of biological chemistry · 2016Article
- Quantitative Proteomics of the Infectious and Replicative Forms of Chlamydia trachomatis.PloS one · 2016Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
19 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlamydia pneumoniae, a human pathogen causing respiratory infections and probably contributing to the development of atherosclerosis and heart disease, is an obligate intracellular parasite which for replication needs to productively interact with and enter human cells. Because of the intrinsic difficulty in working with C. pneumoniae and in the absence of reliable tools for its genetic manipulation, the molecular definition of the chlamydial cell surface is still limited, thus leaving the mechanisms of chlamydial entry largely unknown. In an effort to define the surface protein organization of C. pneumoniae, we have adopted a combined genomic-proteomic approach based on (i) in silico prediction from the available genome sequences of peripherally located proteins, (ii) heterologous expression and purification of selected proteins, (iii) production of mouse immune sera against the recombinant proteins to be used in Western blotting and fluorescence-activated cell sorter (FACS) analyses for the identification of surface antigens, and (iv) mass spectrometry analysis of two-dimensional electrophoresis (2DE) maps of chlamydial protein extracts to confirm the presence of the FACS-positive antigens in the chlamydial cell. Of the 53 FACS-positive sera, 41 recognized a protein species with the expected size on Western blots, and 28 of the 53 antigens shown to be surface-exposed by FACS were identified on 2DE maps of elementary-body extracts. This work represents the first systematic attempt to define surface protein organization in C. pneumoniae.
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