ArticleInfection and immunity2002
Serum stimulates growth of and proteinase secretion by Aspergillus fumigatus.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 62 citations in OpenAlex.
- Glutamate dehydrogenase 2 is required for virulence by facilitating fungal growth in the host hemocoel.Virulence · 2025Article
- Increasing the Sensitivity of Aspergillus Galactomannan ELISA Using Silver Nanoparticle-Based Surface-Enhanced Raman Spectroscopy.Sensors (Basel, Switzerland) · 2025Article
- Precision-cut lung slices in air-liquid interface (PCLS-ALI): A novelbioRxiv : the preprint server for biology · 2024Article
- Powerful cell wall biomass degradation enzymatic system from saprotrophicCell surface (Amsterdam, Netherlands) · 2024Review
- The Aspergillus fumigatus UPR is variably activated across nutrient and host environments and is critical for the establishment of corneal infection.PLoS pathogens · 2023Article
- Fungal Zinc Homeostasis and Its Potential as an Antifungal Target: A Focus on the Human PathogenMicroorganisms · 2022Review
- Review
- Article
- Amphotericin B- and voriconazole-echinocandin combinations against Aspergillus spp.: Effect of serum on inhibitory and fungicidal interactions.Antimicrobial agents and chemotherapy · 2013Article
- Disparate proteome responses of pathogenic and nonpathogenic aspergilli to human serum measured by activity-based protein profiling (ABPP).Molecular & cellular proteomics : MCP · 2013Article
- Inhibitory and fungicidal effects of antifungal drugs against Aspergillus species in the presence of serum.Antimicrobial agents and chemotherapy · 2013Article
- Effects of a defective endoplasmic reticulum-associated degradation pathway on the stress response, virulence, and antifungal drug susceptibility of the mold pathogen Aspergillus fumigatus.Eukaryotic cell · 2013Article
- Production and partial characterization of serine and metallo peptidases secreted by Aspergillus fumigatus Fresenius in submerged and solid state fermentation.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2013Article
- Multiplexed activity-based protein profiling of the human pathogen Aspergillus fumigatus reveals large functional changes upon exposure to human serum.The Journal of biological chemistry · 2012Article
- Expression and secretion of Aspergillus fumigatus proteases are regulated in response to different protein substrates.Fungal biology · 2012Article
- Article
- A secreted serine protease of Paracoccidioides brasiliensis and its interactions with fungal proteins.BMC microbiology · 2010Article
- Secreted Aspergillus fumigatus protease Alp1 degrades human complement proteins C3, C4, and C5.Infection and immunity · 2010Article
- Evaluation of lysine biosynthesis as an antifungal drug target: biochemical characterization of Aspergillus fumigatus homocitrate synthase and virulence studies.Eukaryotic cell · 2010Article
- Transcription factor PrtT controls expression of multiple secreted proteases in the human pathogenic mold Aspergillus fumigatus.Infection and immunity · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serum contains iron-binding proteins, which inhibit the growth of most pathogenic microorganisms, including fungi. The purpose of this research was to investigate the effect of serum on growth of the opportunistic fungal pathogen Aspergillus fumigatus. Supplementing minimal essential medium (MEM) with up to 80% human serum or up to 80% fetal bovine serum (FBS) stimulated growth and increased the amount of A. fumigatus dry biomass approximately fourfold. In addition, a 100-fold increase in proteinase secretion, as measured by azocasein hydrolysis, was observed when 10% human serum or 10% FBS was added to MEM. The fungal proteinases secreted in serum-containing media were shown to degrade (3)H-labeled basal lamina proteins. The factor in serum that stimulated proteinase secretion was larger than 10 kDa and was 85% inactivated when the serum was heated for 30 min at 66 degrees C. The proportions of proteinases of each catalytic class secreted by A. fumigatus in the presence of serum were different from the proportions secreted in media containing single proteins. Proteinase secretion did not result from increased protein concentration in the medium per se because bovine serum albumin (BSA) at a concentration equivalent to the concentration of serum produced only 20% of the proteinase activity per milligram (dry weight) that was produced by FBS. Addition of BSA plus 100 microM FeCl(3) to MEM resulted in the same level of growth as addition of serum, indicating that a combination of nutritional factors in serum may stimulate growth. However, the level of proteinase secretion was still only 30% of the level observed with FBS. These data indicate that serum does not inhibit the growth of A. fumigatus and that the nutrients in serum result in high levels of proteinase secretion, potentially increasing the invasiveness of this species.
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