ArticleThe Journal of physiology2001
Physiological stimuli evoke two forms of endocytosis in bovine chromaffin cells.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 65 citations in OpenAlex.
- A PACAP-activated network for secretion requires coordination of CaMolecular biology of the cell · 2024Article
- 'Full fusion' is not ineluctable during vesicular exocytosis of neurotransmitters by endocrine cells.Proceedings. Mathematical, physical, and engineering sciences · 2017Article
- Myosin light chain kinase facilitates endocytosis of synaptic vesicles at hippocampal boutons.Journal of neurochemistry · 2016Article
- Sustained Exocytosis after Action Potential-Like Stimulation at Low Frequencies in Mouse Chromaffin Cells Depends on a Dynamin-Dependent Fast Endocytotic Process.Frontiers in cellular neuroscience · 2016Article
- Catecholamine exocytosis during low frequency stimulation in mouse adrenal chromaffin cells is primarily asynchronous and controlled by the novel mechanism of Ca2+ syntilla suppression.The Journal of physiology · 2014Article
- Calcineurin is universally involved in vesicle endocytosis at neuronal and nonneuronal secretory cells.Cell reports · 2014Article
- Syndapin 3 modulates fusion pore expansion in mouse neuroendocrine chromaffin cells.American journal of physiology. Cell physiology · 2014Article
- Exocytosis and endocytosis: modes, functions, and coupling mechanisms.Annual review of physiology · 2014Review
- Most vesicles in a central nerve terminal participate in recycling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Functional chromaffin cell plasticity in response to stress: focus on nicotinic, gap junction, and voltage-gated Ca2+ channels.Journal of molecular neuroscience : MN · 2012Review
- Activity-dependent fusion pore expansion regulated by a calcineurin-dependent dynamin-syndapin pathway in mouse adrenal chromaffin cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Mechanistic analysis of massive endocytosis in relation to functionally defined surface membrane domains.The Journal of general physiology · 2011Article
- Massive calcium-activated endocytosis without involvement of classical endocytic proteins.The Journal of general physiology · 2011Article
- Dynamin and myosin regulate differential exocytosis from mouse adrenal chromaffin cells.Cellular and molecular neurobiology · 2010Review
- Rapid endocytosis and vesicle recycling in neuroendocrine cells.Cellular and molecular neurobiology · 2010Review
- Developmental shift to a mechanism of synaptic vesicle endocytosis requiring nanodomain Ca2+.Nature neuroscience · 2010Article
- Loose coupling between calcium channels and sites of exocytosis in chromaffin cells.The Journal of physiology · 2009Article
- The molecular physiology of activity-dependent bulk endocytosis of synaptic vesicles.Journal of neurochemistry · 2009Review
- Enhanced dense core granule function and adrenal hypersecretion in a mouse model of Rett syndrome.The European journal of neuroscience · 2009Article
- Cortical F-actin, the exocytic mode, and neuropeptide release in mouse chromaffin cells is regulated by myristoylated alanine-rich C-kinase substrate and myosin II.Molecular biology of the cell · 2009Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Exocytosis and endocytosis were measured following single, or trains of, simulated action potentials (sAP) in bovine adrenal chromaffin cells. Catecholamine secretion was measured by oxidative amperometry and cell membrane turnover was measured by voltage clamp cell capacitance measurements. 2. The sAPs evoked inward Na(+) and Ca(2+) currents that were statistically identical to those evoked by native action potential waveforms. On average, a single secretory granule underwent fusion following sAP stimulation. An equivalent amount of membrane was then quickly internalised (tau = 560 ms). 3. Stimulation with sAP trains revealed a biphasic relationship between cell firing rate and endocytic activity. At basal stimulus frequencies (single to 0.5 Hz) cells exhibited a robust membrane internalisation that then diminished as firing increased to intermediate levels (1.9 and 6 Hz). However at the higher stimulation rates (10 and 16 Hz) endocytic activity rebounded and was again able to effectively maintain cell surface near pre-stimulus levels. 4. Treatment with cyclosporin A and FK506, inhibitors of the phosphatase calcineurin, left endocytosis characteristics unaltered at the lower basal stimulus levels, but blocked the resurgence in endocytosis seen in control cells at higher sAP frequencies. 5. Based on these findings we propose that, under physiological electrical stimulation, chromaffin cells internalise membrane via two distinct pathways that are separable. One is prevalent at basal stimulus frequencies, is lessened with increased firing, and is insensitive to cyclosporin A and FK506. A second endocytic form is activated by increased firing frequencies, and is selectively blocked by cyclosporin A and FK506.
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