ArticlePlant physiology2001
A slow maturation of a cysteine protease with a granulin domain in the vacuoles of senescing Arabidopsis leaves.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 173 citations in OpenAlex.
- The old nourishes the new: 100 years of leaf senescence studies in Plant Physiology.Plant physiology · 2026Review
- NbRD21 protease controls receptor kinase homeostasis in Nicotiana benthamiana.The New phytologist · 2026Article
- 5-azacytidine affects triticale microspore embryogenesis and is associated with modulation of reactive oxygen species, cysteine protease profiles, and programmed cell death.BMC plant biology · 2026Article
- A secreted citrus protease cleaves an outer membrane protein of the Huanglongbing pathogen.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- RD21-like proteases: key effector hubs in plant-pathogen interactions.Journal of experimental botany · 2025Review
- Genome-wide molecular characterization and expression profiling of the cysteine protease gene family in maize.BMC genomics · 2025Article
- Quantitative Proteomic Analysis of Brassica Napus Reveals Intersections Between Nutrient Deficiency Responses.Plant, cell & environment · 2025Article
- Papain-Like Cysteine Proteases Contribute to Functional Cleavage of Begomoviral V2 Effector Required for Relevant Virulences.Molecular plant pathology · 2025Article
- Pull the fuzes: Processing protein precursors to generate apoplastic danger signals for triggering plant immunity.Plant communications · 2024Review
- TcSERPIN, an inhibitor that interacts with cocoa defense proteins and has biotechnological potential against human pathogens.Frontiers in plant science · 2024Article
- A Novel Senescence-Specific Gene (International journal of molecular sciences · 2022Article
- Transient proteolysis reduction ofFrontiers in plant science · 2022Article
- Plant protease as regulator and signaling molecule for enhancing environmental stress-tolerance.Plant cell reports · 2021Review
- Tobacco BY2 cells expressing recombinant cardosin B as an alternative for production of active milk clotting enzymes.Scientific reports · 2021Article
- Age- and stress-associated C. elegans granulins impair lysosomal function and induce a compensatory HLH-30/TFEB transcriptional response.PLoS genetics · 2019Article
- Papain-like cysteine proteases prepare plant cyclic peptide precursors for cyclization.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Do proteolytic cascades exist in plants?Journal of experimental botany · 2019Review
- Cutting Out the Gaps Between Proteases and Programmed Cell Death.Frontiers in plant science · 2019Article
- A Genotypic Comparison Reveals That the Improvement in Nitrogen Remobilization Efficiency in Oilseed Rape Leaves Is Related to Specific Patterns of Senescence-Associated Protease Activities and Phytohormones.Frontiers in plant science · 2019Article
- Isolation and characterization of a tandem-repeated cysteine protease from the symbiotic dinoflagellate Symbiodinium sp. KB8.PloS one · 2019Article
16 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arabidopsis RD21 is a cysteine protease of the papain family. Unlike other members of the papain family, RD21 has a C-terminal extension sequence composed of two domains, a 2-kD proline-rich domain and a 10-kD domain homologous to animal epithelin/granulin family proteins. The RD21 protein was accumulated as 38- and 33-kD proteins in Arabidopsis leaves. An immunoblot showed that the 38-kD protein had the granulin domain, whereas the 33-kD protein did not. A pulse-chase experiment with Bright-Yellow 2 transformant cells expressing RD21 showed that RD21 was synthesized as a 57-kD precursor and was then slowly processed to make the 33-kD mature protein via the 38-kD intermediate. After a 12-h chase, the 38-kD intermediate was still detected in the cells. These results indicate that the N-terminal propeptide was first removed from the 57-kD precursor, and the C-terminal granulin domain was then slowly removed to yield the 33-kD mature protein. Subcellular fractionation of the Bright-Yellow 2 transformant showed that the intermediate and mature forms of RD21 were localized in the vacuoles. Under the acidic conditions of the vacuolar interior, the intermediate was found to be easily aggregated. The intermediate and the mature protein were accumulated in association with leaf senescence. Taken together, these results indicate that the intermediate of RD21 was accumulated in the vacuoles as an aggregate, and then slowly matured to make a soluble protease by removing the granulin domain during leaf senescence.
Indexed as
Identifiers
11743107PMC133567W2134267576What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.