ArticleThe EMBO journal2001
Mutations in human DNA polymerase eta motif II alter bypass of DNA lesions.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 55 citations in OpenAlex.
- Whole-exome sequencing reveals the impact of UVA light mutagenesis in xeroderma pigmentosum variant human cells.Nucleic acids research · 2020Article
- Increased Processivity, Misincorporation, and Nucleotide Incorporation Efficiency in Sulfolobus solfataricus Dpo4 Thumb Domain Mutants.Applied and environmental microbiology · 2017Article
- Comparative molecular dynamics studies of heterozygous open reading frames of DNA polymerase eta (η) in pathogenic yeast Candida albicans.Scientific reports · 2017Article
- Structural basis of human DNA polymerase η-mediated chemoresistance to cisplatin.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Characterization of Escherichia coli UmuC active-site loops identifies variants that confer UV hypersensitivity.Journal of bacteriology · 2011Article
- A nucleotide binding rectification Brownian ratchet model for translocation of Y-family DNA polymerases.Theoretical biology & medical modelling · 2011Article
- Structure and mechanism of human DNA polymerase eta.Nature · 2010Article
- CyDNA: synthesis and replication of highly Cy-dye substituted DNA by an evolved polymerase.Journal of the American Chemical Society · 2010Article
- Eukaryotic Y-family polymerases bypass a 3-methyl-2'-deoxyadenosine analog in vitro and methyl methanesulfonate-induced DNA damage in vivo.Nucleic acids research · 2008Article
- Effects of accessory proteins on the bypass of a cis-syn thymine-thymine dimer by Saccharomyces cerevisiae DNA polymerase eta.Biochemistry · 2007Article
- Protein tolerance to random amino acid change.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Palm mutants in DNA polymerases alpha and eta alter DNA replication fidelity and translesion activity.Molecular and cellular biology · 2004Article
- Deoxynucleotide triphosphate binding mode conserved in Y family DNA polymerases.Molecular and cellular biology · 2003Article
- Biochemical analysis of active site mutations of human polymerase η.Mutation researchArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Human DNA polymerase eta (hPol eta) is one of the newly identified Y-family of DNA polymerases. These polymerases synthesize past template lesions that are postulated to block replication fork progression. hPol eta accurately bypasses UV-associated cis-syn cyclobutane thymine dimers in vitro and contributes to normal resistance to sunlight-induced skin cancer. We describe here mutational analysis of motif II, a highly conserved sequence, recently reported to reside in the fingers domain and to form part of the active site in Y-family DNA polymerases. We used a yeast-based complementation system to isolate biologically active mutants created by random sequence mutagenesis, synthesized the mutant proteins in vitro and assessed their ability to bypass thymine dimers. The mutability of motif II in 210 active mutants has parallels with natural evolution and identifies Tyr52 and Ala54 as prime candidates for involvement in catalytic activity or bypass. We describe the ability of hPol eta S62G, a mutant polymerase with enhanced activity, to bypass five other site-specific lesions. Our results may serve as a prototype for studying other members of the Y-family DNA polymerases.
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