ArticleThe EMBO journal2001
A dynamic competition between release factor 2 and the tRNA(Sec) decoding UGA at the recoding site of Escherichia coli formate dehydrogenase H.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 41 citations in OpenAlex.
- Stop codon reassignment to tryptophan in members of the bacterial phylumMicrobial genomics · 2026Article
- Bioinformatic Prediction of an tRNAInternational journal of molecular sciences · 2021Article
- On elongation factor eEFSec, its role and mechanism during selenium incorporation into nascent selenoproteins.Biochimica et biophysica acta. General subjects · 2018Review
- A quantitative model for the rate-limiting process of UGA alternative assignments to stop and selenocysteine codons.PLoS computational biology · 2017Article
- Genetic code flexibility in microorganisms: novel mechanisms and impact on physiology.Nature reviews. Microbiology · 2015Review
- Partitioning between recoding and termination at a stop codon-selenocysteine insertion sequence.Nucleic acids research · 2015Article
- Evolving tRNA(Sec) for efficient canonical incorporation of selenocysteine.Journal of the American Chemical Society · 2015Article
- Recoding the genetic code with selenocysteine.Angewandte Chemie (International ed. in English) · 2014Article
- Wobble decoding by the Escherichia coli selenocysteine insertion machinery.Nucleic acids research · 2013Article
- Carbon source-dependent expansion of the genetic code in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The codon specificity of eubacterial release factors is determined by the sequence and size of the recognition loop.RNA (New York, N.Y.) · 2010Article
- Bioinformatic, structural, and functional analyses support release factor-like MTRF1 as a protein able to decode nonstandard stop codons beginning with adenine in vertebrate mitochondria.RNA (New York, N.Y.) · 2010Article
- Factors and selenocysteine insertion sequence requirements for the synthesis of selenoproteins from a gram-positive anaerobe in Escherichia coli.Applied and environmental microbiology · 2008Article
- Assessment of production conditions for efficient use of Escherichia coli in high-yield heterologous recombinant selenoprotein synthesis.Applied and environmental microbiology · 2004Article
- Sequences that direct significant levels of frameshifting are frequent in coding regions of Escherichia coli.The EMBO journal · 2003Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Factors affecting competition between termination and elongation in vivo during translation of the fdhF selenocysteine recoding site (UGA) were studied with wild-type and modified fdhF sequences. Altering sequences surrounding the recoding site UGA without affecting RNA secondary structure indicated that the kinetics of stop signal decoding have a significant influence on selenocysteine incorporation efficiency. The UGA in the wild-type fdhF sequence remains 'visible' to the factor and forms a site-directed cross-link when mRNA stem-loop secondary structure is absent, but not when it is present. The timing of the secondary structure unfolding during translation may be a critical feature of competition between release factor 2 and tRNA(Sec) for decoding UGA. Increasing the cellular concentration of either of these decoding molecules for termination or selenocysteine incorporation showed that they were able to compete for UGA by a kinetic competition that is dynamic and dependent on the Escherichia coli growth rate. The tRNA(Sec)-mediated decoding can compete more effectively for the UGA recoding site at lower growth rates, consistent with anaerobic induction of fdhF expression.
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