ArticleThe EMBO journal2001
Acetylcholine receptors are required for agrin-induced clustering of postsynaptic proteins.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- Zebrafish mutants of the neuromuscular junction: swimming in the gene pool.The journal of physiological sciences : JPS · 2015Review
- The knockdown of αkap alters the postsynaptic apparatus of neuromuscular junctions in living mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Structure and superorganization of acetylcholine receptor-rapsyn complexes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Acetylcholine receptors enable the transport of rapsyn from the Golgi complex to the plasma membrane.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Article
- Article
- Acetylcholine negatively regulates development of the neuromuscular junction through distinct cellular mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Statistical analysis of high-resolution light microscope images reveals effects of cytoskeleton-disrupting drugs on the membrane organization of the nicotinic acetylcholine receptor.The Journal of membrane biology · 2010Article
- Rapsyn interacts with the muscle acetylcholine receptor via alpha-helical domains in the alpha, beta, and epsilon subunit intracellular loops.Neuroscience · 2009Article
- Rapsyn carboxyl terminal domains mediate muscle specific kinase-induced phosphorylation of the muscle acetylcholine receptor.Neuroscience · 2008Article
- Dp71, utrophin and beta-dystroglycan expression and distribution in PC12/L6 cell cocultures.Neuroreport · 2007Article
- Tyrosine phosphatases such as SHP-2 act in a balance with Src-family kinases in stabilization of postsynaptic clusters of acetylcholine receptors.BMC neuroscience · 2007Article
- Overexpression of rapsyn in rat muscle increases acetylcholine receptor levels in chronic experimental autoimmune myasthenia gravis.The American journal of pathology · 2007Article
- Cholesterol and lipid microdomains stabilize the postsynapse at the neuromuscular junction.The EMBO journal · 2006Article
- Induction of myasthenia by immunization against muscle-specific kinase.The Journal of clinical investigation · 2006Article
- Differential dependence of axo-dendritic and axo-somatic GABAergic synapses on GABAA receptors containing the alpha1 subunit in Purkinje cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Src-family kinases stabilize the neuromuscular synapse in vivo via protein interactions, phosphorylation, and cytoskeletal linkage of acetylcholine receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Increased ratio of rapsyn to ACh receptor stabilizes postsynaptic receptors at the mouse neuromuscular synapse.The Journal of physiology · 2005Article
- A single pulse of agrin triggers a pathway that acts to cluster acetylcholine receptors.Molecular and cellular biology · 2004Article
- Acetylcholine receptors direct rapsyn clusters to the neuromuscular synapse in zebrafish.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
We have investigated the role of acetylcholine receptors (AChRs) in an early step of postsynaptic assembly at the neuromuscular synapse, the clustering of postsynaptic proteins induced by nerve-released agrin. To achieve this, we used two variants of C2 myotubes virtually lacking AChRs and C2 cells in which surface AChRs were down-regulated by AChR antibodies. In all cases, agrin caused normal clustering of the agrin receptor component MuSK, alpha-dystrobrevin and utrophin, but failed to aggregate AChRs, alpha- and beta-dystroglycan, syntrophin isoforms and rapsyn, an AChR-anchoring protein necessary for postsynaptic assembly and AChR clustering. In C2 variants, the stability of rapsyn was decreased, whereas in antibody-treated cells, rapsyn efficiently co-localized with remaining AChRs in microaggregates. Upon ectopic injection into myofibers in vivo, rapsyn did not form clusters in the absence of AChRs. These results show that AChRs and rapsyn are interdependent components of a pre-assembled protein complex that is required for agrin-induced clustering of a full set of postsynaptic proteins, thus providing evidence for an active role of AChRs in postsynaptic assembly.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.