ArticleProceedings of the National Academy of Sciences of the United States of America2001
The expression of human alpha -like globin genes in transgenic mice mediated by bacterial artificial chromosome.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 28 citations in OpenAlex.
- Functional validation of mouse tyrosinase non-coding regulatory DNA elements by CRISPR-Cas9-mediated mutagenesis.Nucleic acids research · 2015Article
- Unravelling the world of cis-regulatory elements.Medical & biological engineering & computing · 2007Review
- A general method to modify BACs to generate large recombinant DNA fragments.Molecular biotechnology · 2005Article
- A 191-kb genomic fragment containing the human alpha-globin locus can rescue alpha-thalassemic mice.Mammalian genome : official journal of the International Mammalian Genome Society · 2005Article
- Knockdown of mouse adult beta-globin gene expression in MEL cells by retrovirus vector-mediated RNA interference.Molecular biotechnology · 2004Article
- Complementation of alpha-thalassaemia in alpha-globin knockout mice with a 191 kb transgene containing the human alpha-globin locus.Transgenic research · 2004Article
- Evaluation of optimal expression cassette in retrovirus vector for beta-thalassemia gene therapy.Molecular biotechnology · 2003Article
- The control of expression of the alpha-globin gene cluster.International journal of hematology · 2002Review
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Authors and funding
8 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
After screening a bacterial artificial chromosome of human genomic DNA library with human HS-40, zeta-, alpha-, and theta-globin probes, a 110-kb clone bearing the whole human alpha-globin gene cluster was obtained and rare restriction endonuclease mapping was performed. The bacterial artificial chromosome DNA was isolated, and transgenic mice were generated. Three founders were detected from 35 newborn mice. The copy numbers were 1, 2, and 2, and the expression of human alpha-globin genes in various tissues at different developmental stages in the transgenic mice was assayed. The human alpha-globin mRNA can be detected in bone marrow, kidney, liver, brain, but not in muscle, testis, or thymus. The human zeta-globin genes were switched off, and the alpha-globin genes were switched at day 11.5 in mouse embryo, indicating that developmental stage-specific expression of the alpha-like globin genes was properly regulated. The human alpha-globin mRNA ranged between 17-68% of the endogenous mouse alpha-globin, suggesting that the expression of human alpha-globin genes is integration site-dependent in transgenic mice. The ratio of human alpha(2)- and alpha(1)-globin gene expression in adult transgenic mouse is about 2.5:1 similar to the expression in human.
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