ArticleJournal of bacteriology2002
The active component of the bioemulsifier alasan from Acinetobacter radioresistens KA53 is an OmpA-like protein.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 88 citations in OpenAlex.
- Biosurfactants as novel antibiofilm agents: mechanisms, biomedical applications, and applications in biofilm-associated infections.Archives of microbiology · 2025Review
- Novel Insights Into the Struggle Against Biofilm: The PsyOmp38 Protein From the Antarctic Marine Bacterium Psychrobacter sp. TAE2020.Microbial biotechnology · 2025Article
- Unveiling the structure-emulsifying function relationship of truncated recombinant forms of the SA01-OmpA protein opens up a new vista in bioemulsifiers.Microbiology spectrum · 2024Article
- DNA shuffling to improve crude-water interfacial activity in biosurfactants with OmpA protein ofPeerJ · 2024Article
- A comprehensive review on production of bio-surfactants by bio-degradation of waste carbohydrate feedstocks: an approach towards sustainable development.RSC advances · 2023Review
- Article
- New Provisional Function of OmpA frommSystems · 2021Article
- Designer bioemulsifiers based on combinations of different polysaccharides with the novel emulsifying esterase AXE from Bacillus subtilis CICC 20034.Microbial cell factories · 2019Article
- Marine derived biosurfactants: a vast potential future resource.Biotechnology letters · 2018Review
- Characterization of a novel bioemulsifier from Pseudomonas stutzeri.World journal of microbiology & biotechnology · 2017Article
- The potential biotechnological applications of the exopolysaccharide produced by the halophilic bacterium Halomonas almeriensis.Molecules (Basel, Switzerland) · 2012Article
- Article
- The marine isolate Novosphingobium sp. PP1Y shows specific adaptation to use the aromatic fraction of fuels as the sole carbon and energy source.Microbial ecology · 2011Article
- Proinflammatory effect in whole blood by free soluble bacterial components released from planktonic and biofilm cells.BMC microbiology · 2008Article
- Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis.Nature biotechnology · 2006Article
- Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium.Nucleic acids research · 2004Article
- An exocellular protein from the oil-degrading microbe Acinetobacter venetianus RAG-1 enhances the emulsifying activity of the polymeric bioemulsifier emulsan.Applied and environmental microbiology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bioemulsifier of Acinetobacter radioresistens KA53, referred to as alasan, is a high-molecular-weight complex of polysaccharide and protein. Recently, one of the alasan proteins, with an apparent molecular mass of 45 kDa, was purified and shown to constitute most of the emulsifying activity. The N-terminal sequence of the 45-kDa protein showed high homology to an OmpA-like protein from Acinetobacter spp. In the research described here the gene coding for the 45-kDa protein was cloned, sequenced, and expressed in Escherichia coli. Recombinant protein AlnA (35.77 kDa without the leader sequence) had an amino acid sequence homologous to that of E. coli OmpA and contained 70% of the specific (hydrocarbon-in-water) emulsifying activity of the native 45-kDa protein and 2.4 times that of the alasan complex. In addition to their emulsifying activity, both the native 45-kDa protein and the recombinant AlnA were highly effective in solubilizing phenanthrene, ca. 80 microg per mg of protein, corresponding to 15 to 19 molecules of phenanthrene per molecule of protein. E. coli OmpA had no significant emulsifying or phenanthrene-solubilizing activity. The production of a recombinant surface-active protein (emulsification and solubilization of hydrocarbons in water) from a defined gene makes possible for the first time structure-function studies of a bioemulsan.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.