ArticleJournal of bacteriology2002
Genetic screen yields mutations in genes encoding all known components of the Escherichia coli signal recognition particle pathway.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Reduced ATP-dependent proteolysis of functional proteins during nutrient limitation speeds the return of microbes to a growth state.Science signaling · 2021Article
- Compounds targeting disulfide bond forming enzyme DsbB of Gram-negative bacteria.Nature chemical biology · 2015Article
- Transcriptome dynamics-based operon prediction in prokaryotes.BMC bioinformatics · 2014Article
- Characterization of the secretion pathway of the collagen adhesin EmaA of Aggregatibacter actinomycetemcomitans.Molecular oral microbiology · 2012Article
- Binding Protein-Dependent Uptake of Maltose into Cells via an ATP-Binding Cassette Transporter.EcoSal Plus · 2010Article
- Depletion of the signal recognition particle receptor inactivates ribosomes in Escherichia coli.Journal of bacteriology · 2009Article
- Rational design of a fusion partner for membrane protein expression in E. coli.Protein science : a publication of the Protein Society · 2009Article
- Characterization of conserved bases in 4.5S RNA of Escherichia coli by construction of new F' factors.Journal of bacteriology · 2008Article
- The signal recognition particle (SRP) RNA links conformational changes in the SRP to protein targeting.Molecular biology of the cell · 2007Article
- Export of the pseudopilin XcpT of the Pseudomonas aeruginosa type II secretion system via the signal recognition particle-Sec pathway.Journal of bacteriology · 2007Article
- SRP RNA provides the physiologically essential GTPase activation function in cotranslational protein targeting.RNA (New York, N.Y.) · 2007Article
- The Haloferax volcanii FtsY homolog is critical for haloarchaeal growth but does not require the A domain.Journal of bacteriology · 2005Article
- Use of thioredoxin as a reporter to identify a subset of Escherichia coli signal sequences that promote signal recognition particle-dependent translocation.Journal of bacteriology · 2005Article
- Target-directed proteolysis at the ribosome.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- SecM facilitates translocase function of SecA by localizing its biosynthesis.Genes & development · 2005Article
- Translation arrest of SecM is essential for the basal and regulated expression of SecA.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Secretion defects that activate the phage shock response of Escherichia coli.Journal of bacteriology · 2003Article
- The DsbA signal sequence directs efficient, cotranslational export of passenger proteins to the Escherichia coli periplasm via the signal recognition particle pathway.Journal of bacteriology · 2003Article
- Secretion of LamB-LacZ by the signal recognition particle pathway of Escherichia coli.Journal of bacteriology · 2003Article
- In vivo analysis of an essential archaeal signal recognition particle in its native host.Journal of bacteriology · 2002Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We describe the further utilization of a genetic screen that identifies mutations defective in the assembly of proteins into the Escherichia coli cytoplasmic membrane. The screen yielded mutations in each of the known genes encoding components of the E. coli signal recognition particle pathway: ffh, ffs, and ftsY, which encode Ffh, 4.5S RNA, and FtsY, respectively. In addition, the screen yielded mutations in secM, which is involved in regulating levels of the SecA component of the bacterium's protein export pathway. We used a sensitive assay involving biotinylation to show that all of the mutations caused defects in the membrane insertions of three topologically distinct membrane proteins, AcrB, MalF, and FtsQ. Among the mutations that resulted in membrane protein insertion defects, only the secM mutations also showed defects in the translocation of proteins into the E. coli periplasm. Genetic evidence suggests that the S382T alteration of Ffh affects the interaction between Ffh and 4.5S RNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.