ArticleJournal of bacteriology2002
Regulation of the metC-cysK operon, involved in sulfur metabolism in Lactococcus lactis.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 73 citations in OpenAlex.
- Development ofFrontiers in microbiology · 2020Article
- Phenotypic Diversity ofFrontiers in microbiology · 2018Article
- Methionine-mediated gene expression and characterization of the CmhR regulon inMicrobial genomics · 2016Article
- Stress Physiology of Lactic Acid Bacteria.Microbiology and molecular biology reviews : MMBR · 2016Review
- Cysteine-Mediated Gene Expression and Characterization of the CmbR Regulon inFrontiers in microbiology · 2016Article
- Article
- Exploring Amino Acid Auxotrophy in Bifidobacterium bifidum PRL2010.Frontiers in microbiology · 2015Article
- Fermentation-induced variation in heat and oxidative stress phenotypes of Lactococcus lactis MG1363 reveals transcriptome signatures for robustness.Microbial cell factories · 2014Article
- Metabolic responses of Lactobacillus plantarum strains during fermentation and storage of vegetable and fruit juices.Applied and environmental microbiology · 2014Article
- Article
- Article
- Computational analysis of cysteine and methionine metabolism and its regulation in dairy starter and related bacteria.Journal of bacteriology · 2012Article
- TcyR regulates L-cystine uptake via the TcyABC transporter in Streptococcus mutans.FEMS microbiology letters · 2012Article
- Acid stress-mediated metabolic shift in Lactobacillus sanfranciscensis LSCE1.Applied and environmental microbiology · 2011Article
- Three paralogous LysR-type transcriptional regulators control sulfur amino acid supply in Streptococcus mutans.Journal of bacteriology · 2010Article
- Comparative genomics of enzymes in flavor-forming pathways from amino acids in lactic acid bacteria.Applied and environmental microbiology · 2008Review
- Control of methionine synthesis and uptake by MetR and homocysteine in Streptococcus mutans.Journal of bacteriology · 2007Article
- Identification and functional characterization of the Lactococcus lactis CodY-regulated branched-chain amino acid permease BcaP (CtrA).Journal of bacteriology · 2006Article
- Regulation of the Bacillus subtilis ytmI operon, involved in sulfur metabolism.Journal of bacteriology · 2005Article
- Sulfur amino acid metabolism and its control in Lactococcus lactis IL1403.Journal of bacteriology · 2005Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sulfur metabolism in gram-positive bacteria is poorly characterized. Information on the molecular mechanisms of regulation of genes involved in sulfur metabolism is limited, and no regulator genes have been identified. Here we describe the regulation of the lactococcal metC-cysK operon, encoding a cystathionine beta-lyase (metC) and cysteine synthase (cysK). Its expression was shown to be negatively affected by high concentrations of cysteine, methionine, and glutathione in the culture medium, while sulfur limitation resulted in a high level of expression. Other sulfur sources tested showed no significant effect on metC-cysK gene expression. In addition we found that O-acetyl-l-serine, the substrate of cysteine synthase, was an inducer of the metC-cysK operon. Using a random mutagenesis approach, we identified two genes, cmbR and cmbT, involved in regulation of metC-cysK expression. The cmbT gene is predicted to encode a transport protein, but its precise role in regulation remains unclear. Disruption of cmbT resulted in a two- to threefold reduction of metC-cysK transcription. A 5.7-kb region containing the cmbR gene was cloned and sequenced. The encoded CmbR protein is homologous to the LysR family of regulator proteins and is an activator of the metC-cysK operon. In analogy to CysB from Escherichia coli, we propose that CmbR requires acetylserine to be able to bind the activation sites and subsequently activate transcription of the metC-cysK operon.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.