ArticleMolecular biology of the cell2001
Plasma membrane proton ATPase Pma1p requires raft association for surface delivery in yeast.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
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Who cites it
103 citing papers in PubMed.
- Unremodeled GPI-anchored proteins at the plasma membrane trigger aberrant endocytosis.Life science alliance · 2025Article
- Molecular tipping points in plant cell surface HQuantitative plant biology · 2025Review
- The joint action of yeast eisosomes and membraneless organelles in response to ethanol stress.Heliyon · 2024Article
- Shared and more specific genetic determinants and pathways underlying yeast tolerance to acetic, butyric, and octanoic acids.Microbial cell factories · 2024Article
- Article
- Ergosterol distribution controls surface structure formation and fungal pathogenicity.bioRxiv : the preprint server for biology · 2023Article
- A Genome-Wide Screen Reveals That Endocytic Genes Are Important for Pma1p Asymmetry during Cell Division inInternational journal of molecular sciences · 2022Article
- Self-organization of apical membrane protein sorting in epithelial cells.The FEBS journal · 2022Article
- Giardial lipid rafts share virulence factors with secreted vesicles and participate in parasitic infection in mice.Frontiers in cellular and infection microbiology · 2022Article
- Golgi-Bypass Is a Major Unconventional Route for Translocation to the Plasma Membrane of Non-Apical Membrane Cargoes inFrontiers in cell and developmental biology · 2022Article
- Structure of the hexameric fungal plasma membrane proton pump in its autoinhibited state.Science advances · 2021Article
- Structure and activation mechanism of the hexameric plasma membrane HNature communications · 2021Article
- A novel AST2 mutation generated upon whole-genome transformation of Saccharomyces cerevisiae confers high tolerance to 5-Hydroxymethylfurfural (HMF) and other inhibitors.PLoS genetics · 2021Article
- Phospholipid flippases and Sfk1 are essential for the retention of ergosterol in the plasma membrane.Molecular biology of the cell · 2021Article
- MPK6 Kinase Regulates Plasma Membrane HInternational journal of molecular sciences · 2021Article
- Mitochondrial Targeting Involving Cholesterol-Rich Lipid Rafts in the Mechanism of Action of the Antitumor Ether Lipid and Alkylphospholipid Analog Edelfosine.Pharmaceutics · 2021Review
- Multifunctional cationic surfactants with a labile amide linker as efficient antifungal agents-mechanisms of action.Applied microbiology and biotechnology · 2021Article
- Yeast Sphingolipid-Enriched Domains and Membrane Compartments in the Absence of Mannosyldiinositolphosphorylceramide.Biomolecules · 2020Article
- The Oligomeric State of the Plasma Membrane H⁺-ATPase fromMolecules (Basel, Switzerland) · 2019Article
- Article
43 more citing papers are in PubMed but not listed here.
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3 authors.
Funding
Abstract
Correct sorting of proteins is essential to generate and maintain the identity and function of the different cellular compartments. In this study we demonstrate the role of lipid rafts in biosynthetic delivery of Pma1p, the major plasma membrane proton ATPase, to the cell surface. Disruption of rafts led to mistargeting of Pma1p to the vacuole. Conversely, Pma1-7, an ATPase mutant that is mistargeted to the vacuole, was shown to exhibit impaired raft association. One of the previously identified suppressors, multicopy AST1, not only restored surface delivery but also raft association of Pma1-7. Ast1p, which is a peripheral membrane protein, was found to directly interact with Pma1p inducing its clustering into a SDS/Triton X100-resistant oligomer. We suggest that clustering facilitates partition of Pma1p into rafts and transport to the cell surface.
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