ArticleMolecular biology of the cell2001
Localization of fission yeast type II myosin, Myo2, to the cytokinetic actin ring is regulated by phosphorylation of a C-terminal coiled-coil domain and requires a functional septation initiation network.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- The Roles of Septins in Regulating Fission Yeast Cytokinesis.Journal of fungi (Basel, Switzerland) · 2024Review
- Article
- Involvement of the septation initiation network in events during cytokinesis in fission yeast.Journal of cell science · 2018Article
- Fission yeast myosin Myo2 is down-regulated in actin affinity by light chain phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Assembly and architecture of precursor nodes during fission yeast cytokinesis.The Journal of cell biology · 2011Article
- The fungal type II myosin in Penicillium marneffei, MyoB, is essential for chitin deposition at nascent septation sites but not actin localization.Eukaryotic cell · 2011Article
- Dividing the spoils of growth and the cell cycle: The fission yeast as a model for the study of cytokinesis.Cytoskeleton (Hoboken, N.J.) · 2011Review
- Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site.Molecular cell · 2010Article
- Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast.The Journal of cell biology · 2009Article
- Regulation of fission yeast myosin-II function and contractile ring dynamics by regulatory light-chain and heavy-chain phosphorylation.Molecular biology of the cell · 2009Article
- Characterization of the minimum domain required for targeting budding yeast myosin II to the site of cell division.BMC biology · 2006Article
- Cytokinesis depends on the motor domains of myosin-II in fission yeast but not in budding yeast.Molecular biology of the cell · 2005Article
- Myosin-II reorganization during mitosis is controlled temporally by its dephosphorylation and spatially by Mid1 in fission yeast.The Journal of cell biology · 2004Article
- Mechanism controlling perpendicular alignment of the spindle to the axis of cell division in fission yeast.The EMBO journal · 2004Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Myo2 truncations fused to green fluorescent protein (GFP) defined a C-terminal domain essential for the localization of Myo2 to the cytokinetic actin ring (CAR). The localization domain contained two predicted phosphorylation sites. Mutation of serine 1518 to alanine (S(1518)A) abolished Myo2 localization, whereas Myo2 with a glutamic acid at this position (S(1518)E) localized to the CAR. GFP-Myo2 formed rings in the septation initiation kinase (SIN) mutant cdc7-24 at 25 degrees C but not at 36 degrees C. GFP-Myo2S(1518)E rings persisted at 36 degrees C in cdc7-24 but not in another SIN kinase mutant, sid2-250. To further examine the relationship between Myo2 and the SIN pathway, the chromosomal copy of myo2(+) was fused to GFP (strain myo2-gc). Myo2 ring formation was abolished in the double mutants myo2-gc cdc7.24 and myo2-gc sid2-250 at the restrictive temperature. In contrast, activation of the SIN pathway in the double mutant myo2-gc cdc16-116 resulted in the formation of Myo2 rings which subsequently collapsed at 36 degrees C. We conclude that the SIN pathway that controls septation in fission yeast also regulates Myo2 ring formation and contraction. Cdc7 and Sid2 are involved in ring formation, in the case of Cdc7 by phosphorylation of a single serine residue in the Myo2 tail. Other kinases and/or phosphatases may control ring contraction.
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