ArticleMolecular biology of the cell2001
Distinct roles of frontal and rear cell-substrate adhesions in fibroblast migration.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
55 citing papers in PubMed, 138 citations in OpenAlex.
- HB-EGF enhances collective cell migration via spatial coordination of traction.APL bioengineering · 2026Article
- The role of extracellular matrix stiffness in regulating fibroblast behaviors and disease progression.Frontiers in immunology · 2026Review
- Enhancing cell motility via non-contact capacitively coupled electrostatic field.Scientific reports · 2024Article
- Calpain Small Subunit Mediated Secretion of Galectin-3 Regulates Traction Stress.Biomedicines · 2024Article
- Regulation of cellular contractile force, shape and migration of fibroblasts by oncogenes and Histone deacetylase 6.Frontiers in molecular biosciences · 2023Article
- TheAnatomy & cell biology · 2022Article
- Reverse Plasticity Underlies Rapid Evolution by Clonal Selection within Populations of Fibroblasts Propagated on a Novel Soft Substrate.Molecular biology and evolution · 2021Article
- Effect of vimentin on cell migration in collagen-coated microchannels: A mimetic physiological confined environment.Biomicrofluidics · 2021Article
- Imaging methods in mechanosensing: a historical perspective and visions for the future.Molecular biology of the cell · 2021Review
- Visualizing the Invisible: Advanced Optical Microscopy as a Tool to Measure Biomechanical Forces.Frontiers in cell and developmental biology · 2021Review
- Nuclear size changes caused by local motion of cell boundaries unfold the nuclear lamina and dilate chromatin and intranuclear bodies.Soft matter · 2019Article
- In Situ Subcellular Detachment of Cells Using a Cell-Friendly Photoresist and Spatially Modulated Light.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Biophysics of Cell-Substrate Interactions Under Shear.Frontiers in cell and developmental biology · 2019Review
- Article
- Predicting Collective Migration of Cell Populations Defined by Varying Repolarization Dynamics.Biophysical journal · 2018Article
- Spatial and temporal coordination of traction forces in one-dimensional cell migration.Cell adhesion & migration · 2016Article
- Guidance of Axons by Local Coupling of Retrograde Flow to Point Contact Adhesions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- The Role of Stress Fibers in the Shape Determination Mechanism of Fish Keratocytes.Biophysical journal · 2016Article
- Osteopontin is proteolytically processed by matrix metalloproteinase 9.Canadian journal of physiology and pharmacology · 2015Article
- Mechanotransduction in intervertebral discs.Journal of cellular and molecular medicine · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Cell migration involves complex physical and chemical interactions with the substrate. To probe the mechanical interactions under different regions of migrating 3T3 fibroblasts, we have disrupted cell-substrate adhesions by local application of the GRGDTP peptide, while imaging stress distribution on the substrate with traction force microscopy. Both spontaneous and GRGDTP-induced detachment of the trailing edge caused extensive cell shortening, without changing the overall level of traction forces or the direction of migration. In contrast, disruption of frontal adhesions caused dramatic, global loss of traction forces before any significant shortening of the cell. Although traction forces and cell migration recovered within 10-20 min of transient frontal treatment, persistent treatment with GRGDTP caused the cell to develop traction forces elsewhere and reorient toward a new direction. We conclude that contractile forces of a fibroblast are transmitted to the substrate through two distinct types of adhesions. Leading edge adhesions are unique in their ability to transmit active propulsive forces. Their functions cannot be transferred directly to existing adhesions upon detachment. Trailing end adhesions create passive resistance during cell migration and readily redistribute their loads upon detachment. Our results indicate the distinct nature of mechanical interactions at the leading versus trailing edges, which together generate the mechanical interactions for fibroblast migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.