ArticleMolecular biology of the cell2001
Cvt18/Gsa12 is required for cytoplasm-to-vacuole transport, pexophagy, and autophagy in Saccharomyces cerevisiae and Pichia pastoris.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers, 3 of them syntheses that pooled it.
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Who cites it
95 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)Autophagy · 2021Guideline
- Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).Autophagy · 2016Guideline
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- Vps1 and select autophagic machinery are required for coenzyme Q uptake and trafficking to mitochondria.The Journal of biological chemistry · 2026Article
- The autophagic and non-autophagic functions of theAutophagy · 2026Article
- Genome-wide analysis reveals the importance of histone acetyltransferase Esa1 in transcriptional regulation during nitrogen starvation.bioRxiv : the preprint server for biology · 2026Article
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- Microautophagy: current understanding of its molecular mechanisms and functions.Autophagy reports · 2026Review
- Microautophagy: definition, classification, and the complexity of the underlying mechanisms.Autophagy · 2026Review
- A novel fluorescence-activated cell sorting (FACS)-based screening identified ATG14, the gene required for pexophagy in the methylotrophic yeast.FEMS yeast research · 2024Article
- Atg18 oligomer organization in assembled tubes and on lipid membrane scaffolds.Nature communications · 2023Article
- Coiled-coil-mediated dimerization of Atg16 is required for binding to the PROPPIN Atg21.Open biology · 2023Article
- Roles of Stress Response in Autophagy Processes and Aging-Related Diseases.International journal of molecular sciences · 2023Review
- The signalling lipid PI3,5POpen biology · 2023Article
- Comprehensive analysis of autophagic functions of WIPI family proteins and their implications for the pathogenesis of β-propeller associated neurodegeneration.Human molecular genetics · 2023Article
- Methylarginine efflux in nutrient-deprived yeast mitigates disruption of nitric oxide synthesis.Amino acids · 2023Article
- Analysis of ATG8 Family Members Using LC3-Interacting Regions (LIR)-Based Molecular Traps.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Autophagy promotes cell survival by maintaining NAD levels.Developmental cell · 2022Article
- Conserved Pib2 regions have distinct roles in TORC1 regulation at the vacuole.Journal of cell science · 2022Article
- Review
35 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
Funding
Abstract
Eukaryotic cells have the ability to degrade proteins and organelles by selective and nonselective modes of micro- and macroautophagy. In addition, there exist both constitutive and regulated forms of autophagy. For example, pexophagy is a selective process for the regulated degradation of peroxisomes by autophagy. Our studies have shown that the differing pathways of autophagy have many molecular events in common. In this article, we have identified a new member in the family of autophagy genes. GSA12 in Pichia pastoris and its Saccharomyces cerevisiae counterpart, CVT18, encode a soluble protein with two WD40 domains. We have shown that these proteins are required for pexophagy and autophagy in P. pastoris and the Cvt pathway, autophagy, and pexophagy in S. cerevisiae. In P. pastoris, Gsa12 appears to be required for an early event in pexophagy. That is, the involution of the vacuole or extension of vacuole arms to engulf the peroxisomes does not occur in the gsa12 mutant. Consistent with its role in vacuole engulfment, we have found that this cytosolic protein is also localized to the vacuole surface. Similarly, Cvt18 displays a subcellular localization that distinguishes it from the characterized proteins required for cytoplasm-to-vacuole delivery pathways.
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