ArticleMolecular biology of the cell2001
The NH(2)-terminus of norepinephrine transporter contains a basolateral localization signal for epithelial cells.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Life and Death of Fungal Transporters under the Challenge of Polarity.International journal of molecular sciences · 2020Review
- Translocation of nutrient transporters to cell membrane via Golgi bypass in Aspergillus nidulans.EMBO reports · 2020Article
- Tales of tails in transporters.Open biology · 2019Review
- Dopamine Transporter Amino and Carboxyl Termini Synergistically Contribute to Substrate and Inhibitor Affinities.The Journal of biological chemistry · 2017Article
- Differentiation of rodent behavioral phenotypes and methylphenidate action in sustained and flexible attention tasks.Brain research · 2016Article
- Differential Internalization Rates and Postendocytic Sorting of the Norepinephrine and Dopamine Transporters Are Controlled by Structural Elements in the N Termini.The Journal of biological chemistry · 2016Article
- Chloride requirement for monoamine transporters.Pflugers Archiv : European journal of physiology · 2016Review
- A current view of serotonin transporters.F1000Research · 2016Review
- The N-terminal basolateral targeting signal unlikely acts alone in the differential trafficking of membrane transporters in MDCK cells.Biochemistry · 2013Article
- The N terminus of monoamine transporters is a lever required for the action of amphetamines.The Journal of biological chemistry · 2010Article
- Molecular determinants dictating cell surface expression of the human sodium-dependent vitamin C transporter-2 in human liver cells.American journal of physiology. Gastrointestinal and liver physiology · 2010Article
- Trafficking of dopamine transporters in psychostimulant actions.Seminars in cell & developmental biology · 2009Review
- C-terminal region regulates the functional expression of human noradrenaline transporter splice variants.The Biochemical journal · 2007Article
- Comparison of the monoamine transporters from human and mouse in their sensitivities to psychostimulant drugs.BMC pharmacology · 2006Article
- Serotonin transporters--structure and function.The Journal of membrane biology · 2006Review
- Synaptic uptake and beyond: the sodium- and chloride-dependent neurotransmitter transporter family SLC6.Pflugers Archiv : European journal of physiology · 2004Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When expressed in epithelial cells, dopamine transporter (DAT) was detected predominantly in the apical plasma membrane, whereas norepinephrine transporter (NET) was found in the basolateral membrane, despite 67% overall amino acid sequence identity. To identify possible localization signals responsible for this difference, DAT-NET chimeras were expressed in MDCK cells and localized by immunocytochemistry and transport assays. The results suggested that localization of these transporters in MDCK cells depends on their highly divergent NH(2)-terminal regions. Deletion of the first 58 amino acids of DAT (preceding TM1) did not change its apical localization. However, the replacement of that region with corresponding sequence from NET resulted in localization of the chimeric protein to the basolateral membrane, suggesting that the NH(2)-terminus of NET, which contains two dileucine motifs, contains a basolateral localization signal. Mutation of these leucines to alanines in the context of a basolaterally localized NET/DAT chimera restored transporter localization to the apical membrane, indicating that the dileucine motifs are critical to the basolateral localization signal embodied within the NET NH(2)-terminal region. However, the same mutation in the context of wild-type NET did not disrupt basolateral localization, indicating the presence of additional signals in NET directing its basolateral localization within the plasma membrane.
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