ArticleMolecular and cellular biology2002
v-Src-induced modulation of the calpain-calpastatin proteolytic system regulates transformation.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 108 citations in OpenAlex.
- The roles of intracellular proteolysis in cardiac ischemia-reperfusion injury.Basic research in cardiology · 2023Review
- Possible etiological association of ovine papillomaviruses with bladder tumors in cattle.Virus research · 2023Review
- PPFIBP1 induces glioma cell migration and invasion through FAK/Src/JNK signaling pathway.Cell death & disease · 2021Article
- Effects of Atypical Protein Kinase C Inhibitor (DNDA) on Lung Cancer Proliferation and Migration by PKC-ι/FAK Ubiquitination Through the Cbl-b Pathway.OncoTargets and therapy · 2020Article
- A Tale of Two States: Normal and Transformed, With and Without Rigidity Sensing.Annual review of cell and developmental biology · 2019Review
- 1,4-dihydroxy quininib attenuates growth of colorectal cancer cells and xenografts and regulates the TIE-2 signaling pathway in patient tumours.Oncotarget · 2019Article
- Baicalein inhibits fibronectin-induced epithelial-mesenchymal transition by decreasing activation and upregulation of calpain-2.Cell death & disease · 2019Article
- KSHV reduces autophagy in THP-1 cells and in differentiating monocytes by decreasing CAST/calpastatin and ATG5 expression.Autophagy · 2016Article
- The inhibition of calpains ameliorates vascular restenosis through MMP2/TGF-β1 pathway.Scientific reports · 2016Article
- A protease storm cleaves a cell-cell adhesion molecule in cancer: multiple proteases converge to regulate PTPmu in glioma cells.Journal of cellular biochemistry · 2014Article
- Increased expression of Capn4 is associated with the malignancy of human glioma.CNS neuroscience & therapeutics · 2014Article
- The Critical Role of Calpain in Cell Proliferation.Journal of biomolecular research & therapeutics · 2014Article
- A role for the protein tyrosine phosphatase CD45 in macrophage adhesion through the regulation of paxillin degradation.PloS one · 2013Article
- Characterization of the expression profiles of calpastatin (CAST) gene in chicken.Molecular biology reports · 2012Article
- Article
- Elevated calpain activity in acute myelogenous leukemia correlates with decreased calpastatin expression.Blood cancer journal · 2012Article
- The calpain system and cancer.Nature reviews. Cancer · 2011Review
- Effects of the plasmid-encoded toxin of enteroaggregative Escherichia coli on focal adhesion complexes.FEMS immunology and medical microbiology · 2011Article
- Calpains as potential anti-cancer targets.Expert opinion on therapeutic targets · 2011Review
- Targeted deletion of Capn4 in cells of the chondrocyte lineage impairs chondrocyte proliferation and differentiation.Molecular and cellular biology · 2010Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
v-Src-induced oncogenic transformation is characterized by alterations in cell morphology, adhesion, motility, survival, and proliferation. To further elucidate some of the signaling pathways downstream of v-Src that are responsible for the transformed cell phenotype, we have investigated the role that the calpain-calpastatin proteolytic system plays during oncogenic transformation induced by v-Src. We recently reported that v-Src-induced transformation of chicken embryo fibroblasts is accompanied by calpain-mediated proteolytic cleavage of the focal adhesion kinase (FAK) and disassembly of the focal adhesion complex. In this study we have characterized a positive feedback loop whereby activation of v-Src increases protein synthesis of calpain II, resulting in degradation of its endogenous inhibitor calpastatin. Reconstitution of calpastatin levels by overexpression of exogenous calpastatin suppresses proteolytic cleavage of FAK, morphological transformation, and anchorage-independent growth. Furthermore, calpastatin overexpression represses progression of v-Src-transformed cells through the G(1) stage of the cell cycle, which correlates with decreased pRb phosphorylation and decreased levels of cyclins A and D and cyclin-dependent kinase 2. Calpain 4 knockout fibroblasts also exhibit impaired v-Src-induced morphological transformation and anchorage-independent growth. Thus, modulation of the calpain-calpastatin proteolytic system plays an important role in focal adhesion disassembly, morphological transformation, and cell cycle progression during v-Src-induced cell transformation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.