ArticleMolecular and cellular biology2002
SRm160 splicing coactivator promotes transcript 3'-end cleavage.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 102 citations in OpenAlex.
- Coordination of nuclear RNA processing by speckle-localized kinase TAOK2.Genes & development · 2026Article
- Combining ferroptosis inducers with gemcitabine to enhance treatment efficacy in pancreatic cancer.Cancer & metabolism · 2026Article
- Heterozygous loss of SRRM1 may be associated with neurodevelopmental phenotypes and anomalies in cell growth and neurite morphology.European journal of human genetics : EJHG · 2026Article
- Network Analysis of Osteoarthritis Progression Using a Steiner Minimal Tree Algorithm.Journal of inflammation research · 2024Article
- SAM homeostasis is regulated by CFIeLife · 2021Article
- Proximity to the Promoter and Terminator Regions Regulates the Transcription Enhancement Potential of an Intron.Frontiers in molecular biosciences · 2021Article
- Gene Architecture Facilitates Intron-Mediated Enhancement of Transcription.Frontiers in molecular biosciences · 2021Review
- Mapping the Interactome of the Nuclear Heparan Sulfate Proteoglycan Syndecan-1 in Mesothelioma Cells.Biomolecules · 2020Article
- Recurrent SRSF2 mutations in MDS affect both splicing and NMD.Genes & development · 2020Article
- Daywake, an Anti-siesta Gene Linked to a Splicing-Based Thermostat from an Adjoining Clock Gene.Current biology : CB · 2019Article
- A cell-based splicing reporter system to identify regulators of cis-splicing between adjacent genes.Nucleic acids research · 2019Article
- HITS-CLIP reveals sex-differential RNA binding and alterative splicing regulation of SRm160 in Drosophila.Journal of molecular cell biology · 2019Article
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- Multifaceted Regulation of Gene Expression by the Apoptosis- and Splicing-Associated Protein Complex and Its Components.International journal of biological sciences · 2017Review
- TRAP150 activates splicing in composite terminal exons.Nucleic acids research · 2014Article
- Multifunctional RNA processing protein SRm160 induces apoptosis and regulates eye and genital development in Drosophila.Genetics · 2014Article
- Splicing-coupled 3' end formation requires a terminal splice acceptor site, but not intron excision.Nucleic acids research · 2013Article
- Regulation of mRNA export by the PI3 kinase/AKT signal transduction pathway.Molecular biology of the cell · 2013Article
- Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Individual steps in the processing of pre-mRNA, including 5'-end cap formation, splicing, and 3'-end processing (cleavage and polyadenylation) are highly integrated and can influence one another. In addition, prior splicing can influence downstream steps in gene expression, including export of mRNA from the nucleus. However, the factors and mechanisms coordinating these steps in the maturation of pre-mRNA transcripts are not well understood. In the present study we demonstrate that SRm160 (for serine/arginine repeat-related nuclear matrix protein of 160 kDa), a coactivator of constitutive and exon enhancer-dependent splicing, participates in 3'-end formation. Increased levels of SRm160 promoted the 3'-end cleavage of transcripts both in vivo and in vitro. Remarkably, at high levels in vivo SRm160 activated the 3'-end cleavage and cytoplasmic accumulation of unspliced pre-mRNAs, thereby uncoupling the requirement for splicing to promote the 3'-end formation and nuclear release of these transcripts. Consistent with a role in 3'-end formation coupled to splicing, SRm160 was found to associate specifically with the cleavage polyadenylation specificity factor and to stimulate the 3'-end cleavage of splicing-active pre-mRNAs more efficiently than that of splicing-inactive pre-mRNAs in vitro. The results provide evidence for a role for SRm160 in mRNA 3'-end formation and suggest that the level of this splicing coactivator is important for the proper coordination of pre-mRNA processing events.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.