ArticleMolecular and cellular biology2002
Protection from free beta-tubulin by the beta-tubulin binding protein Rbl2p.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Tubulin isotype regulation maintains asymmetric requirement for α-tubulin over β-tubulin.The Journal of cell biology · 2023Article
- Cooperative stability renders protein complex formation more robust and controllable.Scientific reports · 2022Article
- The good, the bad, and the ugly: Evolutionary and pathological aspects of gene dosage alterations.PLoS genetics · 2021Review
- Modeling the dynamic behaviors of the COPI vesicle formation regulators, the small GTPase Arf1 and its activating Sec7 guanine nucleotide exchange factor GBF1 on Golgi membranes.Molecular biology of the cell · 2021Article
- Colchicine Blocks Tubulin Heterodimer Recycling by Tubulin Cofactors TBCA, TBCB, and TBCE.Frontiers in cell and developmental biology · 2021Article
- Systematic Humanization of the Yeast Cytoskeleton Discerns Functionally Replaceable from Divergent Human Genes.Genetics · 2020Article
- The role of protein complexes in human genetic disease.Protein science : a publication of the Protein Society · 2019Review
- The TOG protein Stu2/XMAP215 interacts covalently and noncovalently with SUMO.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Quantitative nature of overexpression experiments.Molecular biology of the cell · 2015Article
- Identification of dosage-sensitive genes in Saccharomyces cerevisiae using the genetic tug-of-war method.Genome research · 2013Article
- Mitotic spindle form and function.Genetics · 2012Article
- Faithful chaperones.Cellular and molecular life sciences : CMLS · 2011Review
- TBCD links centriologenesis, spindle microtubule dynamics, and midbody abscission in human cells.PloS one · 2010Article
- Article
- Gene and genome duplications: the impact of dosage-sensitivity on the fate of nuclear genes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Review
- Consequences of defective tubulin folding on heterodimer levels, mitosis and spindle morphology in Saccharomyces cerevisiae.Genetics · 2006Article
- Article
- Size and composition of membrane protein clusters predicted by Monte Carlo analysis.European biophysics journal : EBJ · 2004Article
- A novel step in beta-tubulin folding is important for heterodimer formation in Saccharomyces cerevisiae.Genetics · 2003Article
- An alpha-tubulin mutant demonstrates distinguishable functions among the spindle assembly checkpoint genes in Saccharomyces cerevisiae.Genetics · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Free beta-tubulin not in heterodimers with alpha-tubulin can be toxic, disrupting microtubule assembly and function. We are interested in the mechanisms by which cells protect themselves from free beta-tubulin. This study focused specifically on the function of Rbl2p, which, like alpha-tubulin, can rescue cells from free beta-tubulin. In vitro studies of the mammalian homolog of Rbl2p, cofactor A, have suggested that Rbl2p/cofactor A may be involved in tubulin folding. Here we show that Rbl2p becomes essential in cells containing a modest excess of beta-tubulin relative to alpha-tubulin. However, this essential activity of Rbl2p/cofactorA does not depend upon the reactions described by the in vitro assay. Rescue of beta-tubulin toxicity requires a minimal but substoichiometric ratio of Rbl2p to beta-tubulin. The data suggest that Rbl2p binds transiently to free beta-tubulin, which then passes into an aggregated form that is not toxic.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.