ArticleMolecular and cellular biology2002
Mammalian Orc1 protein is selectively released from chromatin and ubiquitinated during the S-to-M transition in the cell division cycle.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
57 citing papers in PubMed, 131 citations in OpenAlex.
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- Cell identity revealed by precise cell cycle state mapping links data modalities.bioRxiv : the preprint server for biology · 2024Article
- Distinct roles of Arabidopsis ORC1 proteins in DNA replication and heterochromatic H3K27me1 deposition.Nature communications · 2023Article
- Pathway analysis of host responses to dengue virus serotype 2 infection and inhibition of viral envelope protein by naringenin fromJournal of biosciences · 2023Article
- Replication initiation: Implications in genome integrity.DNA repair · 2021Review
- Identification andmicroPublication biology · 2021Article
- The E3 ligase RFWD3 stabilizes ORC in a p53-dependent manner.Cell cycle (Georgetown, Tex.) · 2020Article
- Functional Analysis of the Replication Fork Proteome Identifies BET Proteins as PCNA Regulators.Cell reports · 2019Article
- The ORC ubiquitin ligase OBI1 promotes DNA replication origin firing.Nature communications · 2019Article
- Cortical Development and Brain Malformations: Insights From the Differential Regulation of Early Events of DNA Replication.Frontiers in cell and developmental biology · 2019Review
- Review
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation.EMBO reports · 2017Article
- Dynamic regulation of histone H3K9 is linked to the switch between replication and transcription at the Dbf4 origin-promoter locus.Cell cycle (Georgetown, Tex.) · 2016Article
- Selectivity of ORC binding sites and the relation to replication timing, fragile sites, and deletions in cancers.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Presence of the Paternal Pronucleus Assists Embryo in Overcoming Cycloheximide Induced Abnormalities in Zygotic Mitosis.Journal of cellular biochemistry · 2016Article
- Diverged composition and regulation of the Trypanosoma brucei origin recognition complex that mediates DNA replication initiation.Nucleic acids research · 2016Article
- ORC proteins in the mammalian zygote.Cell and tissue research · 2016Review
- Regulating DNA replication in eukarya.Cold Spring Harbor perspectives in biology · 2013Review
- Genomic instability in cancer.Cold Spring Harbor perspectives in biology · 2013Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Previous studies have shown that changes in the affinity of the hamster Orc1 protein for chromatin during the M-to-G(1) transition correlate with the activity of hamster origin recognition complexes (ORCs) and the appearance of prereplication complexes at specific sites. Here we show that Orc1 is selectively released from chromatin as cells enter S phase, converted into a mono- or diubiquitinated form, and then deubiquitinated and re-bound to chromatin during the M-to-G(1) transition. Orc1 is degraded by the 26S proteasome only when released into the cytosol, and peptide additions to Orc1 make it hypersensitive to polyubiquitination. In contrast, Orc2 remains tightly bound to chromatin throughout the cell cycle and is not a substrate for ubiquitination. Since the concentration of Orc1 remains constant throughout the cell cycle, and its half-life in vivo is the same as that of Orc2, ubiquitination of non-chromatin-bound Orc1 presumably facilitates the inactivation of ORCs by sequestering Orc1 during S phase. Thus, in contrast to yeast (Saccharomyces cerevisiae and Schizosaccharomyces pombe), mammalian ORC activity appears to be regulated during each cell cycle through selective dissociation and reassociation of Orc1 from chromatin-bound ORCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.