ArticleMolecular and cellular biology2002
CAK1 promotes meiosis and spore formation in Saccharomyces cerevisiae in a CDC28-independent fashion.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
- Cdk activation by phosphorylation: linking growth signals to cell cycle control.Biochemical Society transactions · 2025Review
- The MAPK homolog, Smk1, promotes assembly of the glucan layer of the spore wall in S. cerevisiae.Yeast (Chichester, England) · 2024Article
- Isc10, an Inhibitor That Links the Anaphase-Promoting Complex to a Meiosis-Specific Mitogen-Activated Protein Kinase.Molecular and cellular biology · 2020Article
- Ssp2 Binding Activates the Smk1 Mitogen-Activated Protein Kinase.Molecular and cellular biology · 2017Article
- Autophosphorylation of the Smk1 MAPK is spatially and temporally regulated by Ssp2 during meiotic development in yeast.Molecular biology of the cell · 2015Article
- Multisite phosphorylation of the Sum1 transcriptional repressor by S-phase kinases controls exit from meiotic prophase in yeast.Molecular and cellular biology · 2014Article
- Activation of the Smk1 mitogen-activated protein kinase by developmentally regulated autophosphorylation.Molecular and cellular biology · 2013Article
- The Sum1/Ndt80 transcriptional switch and commitment to meiosis in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2012Review
- Sporulation in the budding yeast Saccharomyces cerevisiae.Genetics · 2011Review
- The Cdk1 and Ime2 protein kinases trigger exit from meiotic prophase in Saccharomyces cerevisiae by inhibiting the Sum1 transcriptional repressor.Molecular and cellular biology · 2010Article
- A phosphorylation-independent role for the yeast cyclin-dependent kinase activating kinase Cak1.Gene · 2009Article
- The Ime2 protein kinase enhances the disassociation of the Sum1 repressor from middle meiotic promoters.Molecular and cellular biology · 2009Article
- Article
- The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae.Genetics · 2009Article
- The high-osmolarity glycerol response pathway in the human fungal pathogen Candida glabrata strain ATCC 2001 lacks a signaling branch that operates in baker's yeast.Eukaryotic cell · 2007Article
- Function and regulation in MAPK signaling pathways: lessons learned from the yeast Saccharomyces cerevisiae.Biochimica et biophysica acta · 2007Review
- Mechanisms regulating the protein kinases of Saccharomyces cerevisiae.Eukaryotic cell · 2007Review
- Genetic evidence for a SPO1-dependent signaling pathway controlling meiotic progression in yeast.Genetics · 2007Article
- Identification of yin-yang regulators and a phosphorylation consensus for male germ cell-associated kinase (MAK)-related kinase.Molecular and cellular biology · 2006Article
- Ascospore formation in the yeast Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2005Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
CAK1 encodes a protein kinase in Saccharomyces cerevisiae whose sole essential mitotic role is to activate the Cdc28p cyclin-dependent kinase by phosphorylation of threonine-169 in its activation loop. SMK1 encodes a sporulation-specific mitogen-activated protein (MAP) kinase homolog that is required to regulate the postmeiotic events of spore wall assembly. CAK1 was previously identified as a multicopy suppressor of a weakened smk1 mutant and shown to be required for spore wall assembly. Here we show that Smk1p, like other MAP kinases, is phosphorylated in its activation loop and that Smk1p is not activated in a cak1 missense mutant. Strains harboring a hyperactivated allele of CDC28 that is CAK1 independent and that lacks threonine-169 still require CAK1 to activate Smk1p. The data indicate that Cak1p functions upstream of Smk1p by activating a protein kinase other than Cdc28p. We also found that mutants lacking CAK1 are blocked early in meiotic development, as they show substantial delays in premeiotic DNA synthesis and defects in the expression of sporulation-specific genes, including IME1. The early meiotic role of Cak1p, like the postmeiotic role in the Smk1p pathway, is CDC28 independent. The data indicate that Cak1p activates multiple steps in meiotic development through multiple protein kinase targets.
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Registered trials
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