ArticleThe Journal of cell biology2001
The Tlg SNARE complex is required for TGN homotypic fusion.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 44 citations in OpenAlex.
- Developmental program-independent secretory granule degradation in larval salivary gland cells of Drosophila.Traffic (Copenhagen, Denmark) · 2022Article
- The Vps13p-Cdc31p complex is directly required for TGN late endosome transport and TGN homotypic fusion.The Journal of cell biology · 2017Article
- Vps13 and Cdc31/centrin: Puzzling partners in membrane traffic.The Journal of cell biology · 2017Article
- Direct binding of the Kex2p cytosolic tail to the VHS domain of yeast Gga2p facilitates TGN to prevacuolar compartment transport and is regulated by phosphorylation.Molecular biology of the cell · 2013Article
- Importance of the N-terminal domain of the Qb-SNARE Vti1p for different membrane transport steps in the yeast endosomal system.PloS one · 2013Article
- Transcription factor binding to a DNA zip code controls interchromosomal clustering at the nuclear periphery.Developmental cell · 2012Article
- The yeast GRASP Grh1 colocalizes with COPII and is dispensable for organizing the secretory pathway.Traffic (Copenhagen, Denmark) · 2010Review
- Endocytic and secretory traffic in Arabidopsis merge in the trans-Golgi network/early endosome, an independent and highly dynamic organelle.The Plant cell · 2010Article
- Yeast Golgi-localized, gamma-Ear-containing, ADP-ribosylation factor-binding proteins are but adaptor protein-1 is not required for cell-free transport of membrane proteins from the trans-Golgi network to the prevacuolar compartment.Molecular biology of the cell · 2008Article
- Trans-Golgi network and endosome dynamics connect ceramide homeostasis with regulation of the unfolded protein response and TOR signaling in yeast.Molecular biology of the cell · 2008Article
- Soi3p/Rav1p functions at the early endosome to regulate endocytic trafficking to the vacuole and localization of trans-Golgi network transmembrane proteins.Molecular biology of the cell · 2004Article
- SNAP-23 functions in docking/fusion of granules at low Ca2+.Molecular biology of the cell · 2004Article
- i-SNAREs: inhibitory SNAREs that fine-tune the specificity of membrane fusion.The Journal of cell biology · 2004Article
- Deletion of the SNARE vti1b in mice results in the loss of a single SNARE partner, syntaxin 8.Molecular and cellular biology · 2003Article
- Mammalian ykt6 is a neuronal SNARE targeted to a specialized compartment by its profilin-like amino terminal domain.Molecular biology of the cell · 2003Article
- How Tlg2p/syntaxin 16 'snares' Vps45.The EMBO journal · 2002Article
- t-SNARE phosphorylation regulates endocytosis in yeast.Molecular biology of the cell · 2002Article
- A t-SNARE of the endocytic pathway must be activated for fusion.The Journal of cell biology · 2001Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Using a new assay for membrane fusion between late Golgi/endosomal compartments, we have reconstituted a rapid, robust homotypic fusion reaction between membranes containing Kex2p and Ste13p, two enzymes resident in the yeast trans-Golgi network (TGN). Fusion was temperature, ATP, and cytosol dependent. It was inhibited by dilution, Ca+2 chelation, N-ethylmaleimide, and detergent. Coimmunoisolation confirmed that the reaction resulted in cointegration of the two enzymes into the same bilayer. Antibody inhibition experiments coupled with antigen competition indicated a requirement for soluble NSF attachment protein receptor (SNARE) proteins Tlg1p, Tlg2p, and Vti1p in this reaction. Membrane fusion also required the rab protein Vps21p. Vps21p was sufficient if present on either the Kex2p or Ste13p membranes alone, indicative of an inherent symmetry in the reaction. These results identify roles for a Tlg SNARE complex composed of Tlg1p, Tlg2p, Vti1p, and the rab Vps21p in this previously uncharacterized homotypic TGN fusion reaction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.