ArticleThe Journal of cell biology2001
Crystal structure of Sar1-GDP at 1.7 A resolution and the role of the NH2 terminus in ER export.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
61 citing papers in PubMed, 158 citations in OpenAlex.
- Advances in the basic function of the small GTPase SAR1B and its regulatory role in the biological behavior of tumor cells (Review).Molecular and clinical oncology · 2026Review
- Small GTPase ActIvitY ANalyzing (SAIYAN) system: A method to detect GTPase activation in living cells.The Journal of cell biology · 2024Article
- A molten globule ensemble primes Arf1-GDP for the nucleotide switch.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The interaction networks of small rubber particle proteins in the latex ofFrontiers in plant science · 2024Article
- The alarmone ppGpp selectively inhibits the isoform A of the human small GTPase Sar1.Proteins · 2023Article
- Molecular mechanism of GTP binding- and dimerization-induced enhancement of Sar1-mediated membrane remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A deleterious Sar1c variant in rice inhibits export of seed storage proteins from the endoplasmic reticulum.Plant molecular biology · 2023Article
- Cargo selection in endoplasmic reticulum-to-Golgi transport and relevant diseases.The Journal of clinical investigation · 2023Review
- Circularized fluorescent nanodiscs for probing protein-lipid interactions.Communications biology · 2022Article
- Probing intracellular vesicle trafficking and membrane remodelling by cryo-EM.Journal of structural biology · 2022Review
- Membrane contact probability: An essential and predictive character for the structural and functional studies of membrane proteins.PLoS computational biology · 2022Article
- Article
- Structural basis for the initiation of COPII vesicle biogenesis.Structure (London, England : 1993) · 2021Article
- Structural basis of TRAPPIII-mediated Rab1 activation.The EMBO journal · 2021Article
- Molecular mechanisms that regulate export of the planar cell-polarity protein Frizzled-6 out of the endoplasmic reticulum.The Journal of biological chemistry · 2020Article
- Structural mechanism of a Rag GTPase activation checkpoint by the lysosomal folliculin complex.Science (New York, N.Y.) · 2019Article
- COPII-mediated trafficking at the ER/ERGIC interface.Traffic (Copenhagen, Denmark) · 2019Review
- Chloroplast vesicle transport.Photosynthesis research · 2018Review
- Baicalein Targets GTPase-Mediated Autophagy to Eliminate Liver Tumor-Initiating Stem Cell-Like Cells Resistant to mTORC1 Inhibition.Hepatology (Baltimore, Md.) · 2018Article
- Flexibility of the Sec13/31 cage is influenced by the Sec31 C-terminal disordered domain.Journal of structural biology · 2018Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
The Sar1 GTPase is an essential component of COPII vesicle coats involved in export of cargo from the ER. We report the 1.7-A structure of Sar1 and find that consistent with the sequence divergence of Sar1 from Arf family GTPases, Sar1 is structurally distinct. In particular, we show that the Sar1 NH2 terminus contains two regions: an NH2-terminal extension containing an evolutionary conserved hydrophobic motif that facilitates membrane recruitment and activation by the mammalian Sec12 guanine nucleotide exchange factor, and an alpha1' amphipathic helix that contributes to interaction with the Sec23/24 complex that is responsible for cargo selection during ER export. We propose that the hydrophobic Sar1 NH2-terminal activation/recruitment motif, in conjunction with the alpha1' helix, mediates the initial steps in COPII coat assembly for export from the ER.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.