ArticleThe Journal of cell biology2001
Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 56 citations in OpenAlex.
- Pre-ribosomal particles from nucleoli to cytoplasm.Nucleus (Austin, Tex.) · 2024Review
- Misregulation of Nucleoporins 98 and 96 leads to defects in protein synthesis that promote hallmarks of tumorigenesis.Disease models & mechanisms · 2022Article
- Nuclear export of the pre-60S ribosomal subunit through single nuclear pores observed in real time.Nature communications · 2021Article
- Visualization of Chromatin in the Yeast Nucleus and Nucleolus Using Hyperosmotic Shock.International journal of molecular sciences · 2021Article
- The path of pre-ribosomes through the nuclear pore complex revealed by electron tomography.Nature communications · 2019Article
- Cryo-ET reveals the macromolecular reorganization ofProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Poly(A)-specific ribonuclease regulates the processing of small-subunit rRNAs in human cells.Nucleic acids research · 2017Article
- Nuclear Export of Pre-Ribosomal Subunits Requires Dbp5, but Not as an RNA-Helicase as for mRNA Export.PloS one · 2016Article
- Crystal structure of the N-terminal domain of Nup358/RanBP2.Journal of molecular biology · 2012Article
- Article
- Article
- Maturation of eukaryotic ribosomes: acquisition of functionality.Trends in biochemical sciences · 2010Review
- Immature small ribosomal subunits can engage in translation initiation in Saccharomyces cerevisiae.The EMBO journal · 2010Article
- Characterization of the nuclear and nucleolar localization signals of bovine herpesvirus-1 infected cell protein 27.Virus research · 2009Article
- Distinct cytoplasmic maturation steps of 40S ribosomal subunit precursors require hRio2.The Journal of cell biology · 2009Article
- Nuclear export competence of pre-40S subunits in fission yeast requires the ribosomal protein Rps2.Nucleic acids research · 2008Article
- Arx1 is a nuclear export receptor for the 60S ribosomal subunit in yeast.Molecular biology of the cell · 2008Article
- Crucial role of Bysl in mammalian preimplantation development as an integral factor for 40S ribosome biogenesis.Molecular and cellular biology · 2007Article
- Nup153 affects entry of messenger and ribosomal ribonucleoproteins into the nuclear basket during export.Molecular biology of the cell · 2005Article
- Nuclear export and cytoplasmic processing of precursors to the 40S ribosomal subunits in mammalian cells.The EMBO journal · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To study the nuclear export of preribosomes, ribosomal RNAs were detected by in situ hybridization using fluorescence and EM, in the yeast Saccharomyces cerevisiae. In wild-type cells, semiquantitative analysis shows that the distributions of pre-40S and pre-60S particles in the nucleolus and the nucleoplasm are distinct, indicating uncoordinated transport of the two subunits within the nucleus. In cells defective for the activity of the GTPase Gsp1p/Ran, ribosomal precursors accumulate in the whole nucleus. This phenotype is reproduced with pre-60S particles in cells defective in pre-rRNA processing, whereas pre-40S particles only accumulate in the nucleolus, suggesting a tight control of the exit of the small subunit from the nucleolus. Examination of nucleoporin mutants reveals that preribosome nuclear export requires the Nup82p-Nup159p-Nsp1p complex. In contrast, mutations in the nucleoporins forming the Nup84p complex yield very mild or no nuclear accumulation of preribosome. Interestingly, domains of Nup159p required for mRNP trafficking are not necessary for preribosome export. Furthermore, the RNA helicase Dbp5p and the protein Gle1p, which interact with Nup159p and are involved in mRNP trafficking, are dispensable for ribosomal transport. Thus, the Nup82p-Nup159p-Nsp1p nucleoporin complex is part of the nuclear export pathways of preribosomes and mRNPs, but with distinct functions in these two processes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.