ArticleGenome biology2001
Detection of chromosomes tagged with green fluorescent protein in live Arabidopsis thaliana plants.
Article in Genome biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
- Technologies for engineering repetitive DNA.Quantitative biology (Beijing, China) · 2026Review
- Review
- Decrosslinking enables visualization of RNA-guided endonuclease-in situ labeling signals for DNA sequences in plant tissues.Journal of experimental botany · 2020Article
- Development of a new fluorescent reporter:operator system: location of AraC regulated genes in Escherichia coli K-12.BMC microbiology · 2017Article
- Live-cell CRISPR imaging in plants reveals dynamic telomere movements.The Plant journal : for cell and molecular biology · 2017Article
- Visualization of specific repetitive genomic sequences with fluorescent TALEs in Arabidopsis thaliana.Journal of experimental botany · 2016Article
- Coming to terms with chromatin structure.Chromosoma · 2016Review
- Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research.International journal of molecular sciences · 2015Review
- Chromatin associations in Arabidopsis interphase nuclei.Frontiers in genetics · 2014Article
- Decoding the role of chromatin architecture in development: coming closer to the end of the tunnel.Frontiers in plant science · 2014Review
- De novo generation of plant centromeres at tandem repeats.Chromosoma · 2013Article
- Article
- Binding of lac repressor-GFP fusion protein to lac operator sites inserted in the tobacco chloroplast genome examined by chromatin immunoprecipitation.Nucleic acids research · 2010Article
- Article
- Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings.Plant methods · 2008Article
- Live cell imaging of repetitive DNA sequences via GFP-tagged polydactyl zinc finger proteins.Nucleic acids research · 2007Article
- Sister chromatids are often incompletely aligned in meristematic and endopolyploid interphase nuclei of Arabidopsis thaliana.Genetics · 2006Article
- Use of two-color fluorescence-tagged transgenes to study interphase chromosomes in living plants.Plant physiology · 2005Article
- Cell type-specific characterization of nuclear DNA contents within complex tissues and organs.Plant methods · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStructural and dynamic studies of chromosomes tagged with green fluorescent protein (GFP) in yeast and cultured animal cells have revealed some surprises. Although this technology can be very powerful, only a few studies using this approach with developed multicellular systems have been reported for the study of chromatin behavior in situ.
resultsWe established vectors and conditions to visualize tagged loci stably inserted in the Arabidopsis genome via GFP fused to a bacterial DNA-binding protein. Using this system, three-dimensional coordinates for tagged loci within nuclei from cells of a live plant can be directly determined with concomitant visualization of the position of the nucleolus. Chromosome polyploidization in epidermal cells at the elongation zone of the root in transgenic plants can be visualized in situ using this technique.
conclusionWe have established that GFP fusion with DNA-binding proteins can be used in conjunction with concatameric binding-site arrays to track genomic loci in living Arabidopsis plants. It should now be feasible to study the mechanisms of organization and dynamics of chromatin in specific cell types during various times of plant development, taking advantage of the well developed genetic systems and resources available for Arabidopsis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.