ArticleClinical and experimental immunology2001
Endothelial tissue factor stimulation by proteinase 3 and elastase.
Article in Clinical and experimental immunology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
- Unraveling NETs in Sepsis: From Cellular Mechanisms to Clinical Relevance.International journal of molecular sciences · 2025Review
- Neutrophil extracellular traps facilitate cancer metastasis: cellular mechanisms and therapeutic strategies.Journal of cancer research and clinical oncology · 2023Review
- Neutrophil, neutrophil extracellular traps and endothelial cell dysfunction in sepsis.Clinical and translational medicine · 2023Review
- Role of neutrophils, platelets, and extracellular vesicles and their interactions in COVID-19-associated thrombopathy.Journal of thrombosis and haemostasis : JTH · 2022Review
- The recombinant plant Bauhinia bauhinioides elastase inhibitor reduces rat thrombus without alterations in hemostatic parameters.Scientific reports · 2021Article
- Plaque Erosion: A Distinctive Pathological Mechanism of Acute Coronary Syndrome.Frontiers in cardiovascular medicine · 2021Review
- Neutrophil Extracellular Traps Induce Endothelial Cell Activation and Tissue Factor Production Through Interleukin-1α and Cathepsin G.Arteriosclerosis, thrombosis, and vascular biology · 2018Article
- Mechanisms of erosion of atherosclerotic plaques.Current opinion in lipidology · 2017Review
- Positive feedback loops for factor V and factor VII activation supply sensitivity to local surface tissue factor density during blood coagulation.Biophysical journal · 2011Article
- Tissue factor, blood coagulation, and beyond: an overview.International journal of inflammation · 2011Article
- Identification and validation of urinary biomarkers for differential diagnosis and evaluation of therapeutic intervention in anti-neutrophil cytoplasmic antibody-associated vasculitis.Molecular & cellular proteomics : MCP · 2009Article
- Antibodies to selected minor target antigens in patients with anti-neutrophil cytoplasmic antibodies (ANCA).Clinical and experimental immunology · 2007Article
- Anti-endothelial cell antibodies (AECA) in patients with propylthiouracil (PTU)-induced ANCA positive vasculitis are associated with disease activity.Clinical and experimental immunology · 2005Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In ANCA-associated vasculitis the activation of primed leucocytes by autoantibodies with subsequent release of proteases such as myeloperoxidase (MPO), proteinase 3 (PR3) and elastase is thought to play an important pathogenetic role. Whether these proteases contribute to the vascular lesions by stimulating the procoagulant activity of these cells is unknown. Tissue factor (TF) expression and activity were investigated in human umbilical vein endothelial cells after stimulation with MPO, PR3 and elastase. TF activity was measured using a one-stage clotting assay. Polyclonal antibodies to TF were used to prove specificity. TF mRNA was detected by reverse transcriptase-polymerase chain reaction. PR3 and elastase led to a significant increase in TF mRNA expression and increased activity. The stimulation was not mediated by IL-1. The stimulatory effect of PR3 did not depend on its proteolytic activity (no inhibition by alpha-1-antitrypsin), whereas the effect of elastase was blocked by alpha-1-antitrypsin. MPO had no effect on TF activity. These results show that PR3 and elastase stimulate TF expression in human endothelial cells. In ANCA-associated vasculitis the increased release of proteases may contribute to the development of microthrombi and consecutive necrosis.
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