ArticleThe Biochemical journal2001
Phospholipase D1b and D2a generate structurally identical phosphatidic acid species in mammalian cells.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 72 citations in OpenAlex.
- Mammalian START-like phosphatidylinositol transfer proteins - Physiological perspectives and roles in cancer biology.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2024Review
- Phosphatidic Acid Stimulates Myoblast Proliferation through Interaction with LPA1 and LPA2 Receptors.International journal of molecular sciences · 2021Article
- Beyond Lipid Signaling: Pleiotropic Effects of Diacylglycerol Kinases in Cellular Signaling.International journal of molecular sciences · 2020Review
- Human PLD structures enable drug design and characterization of isoenzyme selectivity.Nature chemical biology · 2020Article
- Mammalian phospholipase D: Function, and therapeutics.Progress in lipid research · 2020Review
- Phospholipase D and Choline Metabolism.Handbook of experimental pharmacology · 2020Review
- Simulated microgravity inhibits C2C12 myogenesis via phospholipase D2-induced Akt/FOXO1 regulation.Scientific reports · 2019Article
- Review
- Clickable Substrate Mimics Enable Imaging of Phospholipase D Activity.ACS central science · 2017Article
- Features of the Phosphatidylinositol Cycle and its Role in Signal Transduction.The Journal of membrane biology · 2017Review
- HS1BP3 negatively regulates autophagy by modulation of phosphatidic acid levels.Nature communications · 2016Article
- Phospholipase D1 deficiency in mice causes nonalcoholic fatty liver disease via an autophagy defect.Scientific reports · 2016Article
- PLD1 regulates adipogenic differentiation through mTOR - IRS-1 phosphorylation at serine 636/639.Scientific reports · 2016Article
- Rapid mitogenic regulation of the mTORC1 inhibitor, DEPTOR, by phosphatidic acid.Molecular cell · 2015Article
- Biochemical characterization of a Pseudomonas aeruginosa phospholipase D.Biochemistry · 2015Article
- Phorbol ester stimulates ethanolamine release from the metastatic basal prostate cancer cell line PC3 but not from prostate epithelial cell lines LNCaP and P4E6.British journal of cancer · 2014Article
- Phospholipase D signaling pathways and phosphatidic acid as therapeutic targets in cancer.Pharmacological reviews · 2014Review
- Caveolae regulate the nanoscale organization of the plasma membrane to remotely control Ras signaling.The Journal of cell biology · 2014Article
- Activation of Src and release of intracellular calcium by phosphatidic acid during Xenopus laevis fertilization.Developmental biology · 2014Article
- Phospholipase D2 is involved in the formation of Golgi tubules and ArfGAP1 recruitment.PloS one · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian cells contain different phospholipase D enzymes (PLDs) whose distinct physiological roles are poorly understood and whose products have not been characterized. The development of porcine aortic endothelial (PAE) cell lines able to overexpress PLD-1b or -2a under the control of an inducible promoter has enabled us to characterize both the substrate specificity and the phosphatidic acid (PtdOH) product of these enzymes under controlled conditions. Liquid chromatography-MS analysis showed that PLD1b- and PLD2a-transfected PAE cells, as well as COS7 and Rat1 cells, generate similar PtdOH and, in the presence of butan-1-ol, phosphatidylbutanol (PtdBut) profiles, enriched in mono- and di-unsaturated species, in particular 16:0/18:1. Although PtdBut mass increased, the species profile did not change in cells stimulated with ATP or PMA. Overexpression of PLD made little difference to basal or stimulated PtdBut formation, indicating that activity is tightly regulated in vivo and that factors other than just PLD protein levels limit hydrolytic function. In vitro assays using PLD-enriched lysates showed that the enzyme could utilize both phosphatidylcholine and, much less efficiently, phosphatidylethanolamine, with slight selectivity towards mono- and di-unsaturated species. Phosphatidylinositol was not a substrate. Thus PLD1b and PLD2a hydrolyse a structurally similar substrate pool to generate an identical PtdOH product enriched in mono- and di-unsaturated species that we propose to function as the intracellular messenger forms of this lipid.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.