ArticleBMC neuroscience2001
Generation of choline for acetylcholine synthesis by phospholipase D isoforms.
Article in BMC neuroscience, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Choline: An Essential Nutrient for Skeletal Muscle.Nutrients · 2020Pooled it
- A lifespan staging model of basal forebrain cholinergic vulnerability.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2026Review
- Liver and Skeletal Muscle Metabolome Characterization in Peripartal Dairy Cows Fed Rumen-Protected Methionine or Rumen-Protected Choline.Animals : an open access journal from MDPI · 2026Article
- Whole brain MR spectroscopy in patients with anti-NMDAR encephalitis.Frontiers in neurology · 2026Article
- Lipid Deposition in Skeletal Muscle Tissues and Its Correlation with Intra-Abdominal Fat: A Pilot Investigation in Type 2 Diabetes Mellitus.Metabolites · 2025Article
- Effect of Fangxia-Dihuang Decoction on doxorubicin-induced cognitive impairment in breast cancer animal model.Frontiers in oncology · 2025Article
- Role of bone marrow mesenchymal stem cell-derived exosomes in reducing neurotoxicity and depression-like behaviors induced by doxorubicin in rats.Toxicology research · 2024Article
- Neurotransmitter acetylcholine mediates the mummification ofApplied and environmental microbiology · 2024Article
- Exploring the Gut Microbiota-Muscle Axis in Duchenne Muscular Dystrophy.International journal of molecular sciences · 2024Review
- Radiopharmaceuticals for Skeletal Muscle PET Imaging.International journal of molecular sciences · 2024Review
- Alpha-Lipoic Acid Ameliorates Doxorubicin-Induced Cognitive Impairments by Modulating Neuroinflammation and Oxidative Stress via NRF-2/HO-1 Signaling Pathway in the Rat Hippocampus.Neurochemical research · 2023Article
- Feasibility ofDiagnostics (Basel, Switzerland) · 2022Article
- Doxorubicin-Induced Cognitive Impairment: The Mechanistic Insights.Frontiers in oncology · 2021Review
- Article
- Protective effects of 7,8-dihydroxyflavone on neuropathological and neurochemical changes in a mouse model of Alzheimer's disease.European journal of pharmacology · 2018Article
- PRMT8 as a phospholipase regulates Purkinje cell dendritic arborization and motor coordination.Science advances · 2015Article
- OX1 orexin/hypocretin receptor activation of phospholipase D.British journal of pharmacology · 2012Article
- Choline transporter 1 maintains cholinergic function in choline acetyltransferase haploinsufficiency.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Phospholipase D and phosphatidate phosphohydrolase activities in rat cerebellum during aging.Lipids · 2004Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundIn cholinergic neurons, the hydrolysis of phosphatidylcholine (PC) by a phospholipase D (PLD)-type enzyme generates some of the precursor choline used for the synthesis of the neurotransmitter acetylcholine (ACh). We sought to determine the molecular identity of the relevant PLD using murine basal forebrain cholinergic SN56 cells in which the expression and activity of the two PLD isoforms, PLD1 and PLD2, were experimentally modified. ACh levels were examined in cells incubated in a choline-free medium, to ensure that their ACh was synthesized entirely from intracellular choline.
resultsPLD2, but not PLD1, mRNA and protein were detected in these cells and endogenous PLD activity and ACh synthesis were stimulated by phorbol 12-myristate 13-acetate (PMA). Introduction of a PLD2 antisense oligonucleotide into the cells reduced PLD2 mRNA and protein expression by approximately 30%. The PLD2 antisense oligomer similarly reduced basal- and PMA-stimulated PLD activity and ACh levels. Overexpression of mouse PLD2 by transient transfection increased basal- (by 74%) and PMA-stimulated (by 3.2-fold) PLD activity. Moreover, PLD2 transfection increased ACh levels by 26% in the absence of PMA and by 2.1-fold in the presence of PMA. Overexpression of human PLD1 by transient transfection increased PLD activity by 4.6-fold and ACh synthesis by 2.3-fold in the presence of PMA as compared to controls.
conclusionsThese data identify PLD2 as the endogenous enzyme that hydrolyzes PC to generate choline for ACh synthesis in cholinergic cells, and indicate that in a model system choline generated by PLD1 may also be used for this purpose.
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