ArticleThe American journal of pathology2001
Simultaneous evaluation of T- and B-cell clonality, t(11;14) and t(14;18), in a single reaction by a four-color multiplex polymerase chain reaction assay and automated high-resolution fragment analysis: a method for the rapid molecular diagnosis of lymphoproliferative disorders applicable to fresh frozen and formalin-fixed, paraffin-embedded tissues, blood, and bone marrow aspirates.
Article in The American journal of pathology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Case report: Histiocytic sarcoma as transdifferentiation of a marginal zone lymphoma-a case presentation based onFrontiers in oncology · 2025Article
- Comparative analysis of primer sets for the assessment of clonality in feline lymphomas.Frontiers in veterinary science · 2024Article
- Case report: 'Atypical Richter transformation from CLL-type monoclonal B-cell lymphocytosis into Burkitt lymphoma in a treatment naïve patient'.Frontiers in oncology · 2024Article
- Case Report: mRNA vaccination-mediated STAT3 overactivation with agranulocytosis and clonal T-LGL expansion.Frontiers in immunology · 2023Article
- Deciphering the genetic landscape of pulmonary lymphomas.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2021Article
- Traumatic ulcerative granuloma with stromal eosinophilia - clinical case report, literature review, and differential diagnosis.World journal of surgical oncology · 2019Review
- ALK-negative anaplastic large cell lymphoma arising in the thrombus of an aortic prosthesis preceeded by clonally related lymphomatoid papulosis.Virchows Archiv : an international journal of pathology · 2019Article
- High-throughput sequencing of nodal marginal zone lymphomas identifies recurrent BRAF mutations.Leukemia · 2018Article
- Genetic background and evolution of relapses in aggressive B-cell lymphomas.Haematologica · 2017Review
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- Relapsed angioimmunoblastic T-cell lymphoma with acquired expression of CD20: a case report and review of the literature.BMC clinical pathology · 2013Article
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- Composite mantle cell lymphoma and chronic lymphocytic leukemia/small lymphocytic lymphoma: a clinicopathologic and molecular study.Human pathology · 2013Review
- Pathophysiology and molecular aspects of diffuse large B-cell lymphoma.Revista brasileira de hematologia e hemoterapia · 2012Article
- Molecular characteristics of mantle cell lymphoma presenting with clonal plasma cell component.The American journal of surgical pathology · 2011Article
- Assay design affects the interpretation of T-cell receptor gamma gene rearrangements: comparison of the performance of a one-tube assay with the BIOMED-2-based TCRG gene clonality assay.The Journal of molecular diagnostics : JMD · 2010Article
- Different proliferative and survival capacity of CLL-cells in a newly established in vitro model for pseudofollicles.Leukemia · 2009Article
- A patient with testicular pseudolymphoma - a rare condition mimicking malignancy: a case report.Journal of medical case reports · 2007Article
- The distribution of gene segments in T-cell receptor gamma gene rearrangements demonstrates the need for multiple primer sets.The Journal of molecular diagnostics : JMD · 2003Article
- Rapid and accurate detection of monoclonal immunoglobulin heavy chain gene rearrangement by DNA melting curve analysis in the LightCycler System.The Journal of molecular diagnostics : JMD · 2002Article
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3 authors.
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Abstract
Current polymerase chain reaction (PCR) methods for the molecular diagnosis of B- and T-cell lymphomas by determination of clonality of immunoglobulin heavy chain (IgH) and T-cell receptor-gamma rearrangements and by detection of the chromosomal translocations t(14;18) and t(11;14), require several laborious and costly PCR assays for each of these diagnostic tests. We have developed a multiplex PCR assay for the simultaneous determination of B- and T-cell clonality and the detection of the chromosomal translocations t(14;18) and t(11;14) in a single reaction, using four-color fluorescence and automated high-resolution fragment analysis. The 26 primers combined in the multiplex PCR correspond to the sequences of >90% of the 69 variables and 6 join IgH genes and 100% of the T-cell receptor-gamma variables and join genes that could participate in the respective rearrangements. In addition, they detect the major and the minor breakpoint regions of the t(14;18) and the major breakpoint region of the t(11;14), and amplify the beta-globin gene as an internal control. The specificity of the multiplex PCR was confirmed by analysis of 39 T-cell lymphomas and 58 B-cell lymphomas, including 11 mantle cell lymphomas bearing the t(11;14) and 25 follicular lymphomas bearing the t(14;18), with known rearrangements and/or translocations. Fifteen samples of reactive lymphadenitis remained negative.
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