ArticleNucleic acids research2001
Combinatorial interactions of two amino acids with a single base pair define target site specificity in plant dimeric homeodomain proteins.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 67 citations in OpenAlex.
- Domain characteristics, classification and expression profiles in response to various abiotic stresses of four HD-Zip subfamilies in tea plant.BMC plant biology · 2025Article
- Genome-wide identification of the HD-ZIP IV gene family inFrontiers in plant science · 2025Article
- Genome-Wide Identification, Characterization, and Expression Analysis of the HD-Zip Gene Family inGenes · 2024Article
- Evolutionary Consequences of Functional and Regulatory Divergence of HD-Zip I Transcription Factors as a Source of Diversity in Protein Interaction Networks in Plants.Journal of molecular evolution · 2023Review
- The START domain mediates Arabidopsis GLABRA2 dimerization and turnover independently of homeodomain DNA binding.Plant physiology · 2022Article
- Genome-wide characterization and expression profiling of Eucalyptus grandis HD-Zip gene family in response to salt and temperature stress.BMC plant biology · 2020Article
- Patterning a Leaf by Establishing Polarities.Frontiers in plant science · 2020Review
- Genome-wide characterization and expression analysis of the HD-Zip gene family in response to drought and salinity stresses in sesame.BMC genomics · 2019Article
- Wheat wounding-responsive HD-Zip IV transcription factor GL7 is predominantly expressed in grain and activates genes encoding defensins.Plant molecular biology · 2019Article
- Multiple Links between HD-Zip Proteins and Hormone Networks.International journal of molecular sciences · 2018Review
- Multiple Pathways in the Control of the Shade Avoidance Response.Plants (Basel, Switzerland) · 2018Review
- Genome-Wide Investigation and Expression Profiling of HD-Zip Transcription Factors in Foxtail Millet (BioMed research international · 2018Article
- Arabidopsis homeodomain-leucine zipper IV proteins promote stomatal development and ectopically induce stomata beyond the epidermis.Development (Cambridge, England) · 2013Article
- Role of Homeodomain leucine zipper (HD-Zip) IV transcription factors in plant development and plant protection from deleterious environmental factors.International journal of molecular sciences · 2013Review
- Uncharacterized conserved motifs outside the HD-Zip domain in HD-Zip subfamily I transcription factors; a potential source of functional diversity.BMC plant biology · 2011Article
- Overexpression of the epidermis-specific homeodomain-leucine zipper IV transcription factor Outer Cell Layer1 in maize identifies target genes involved in lipid metabolism and cuticle biosynthesis.Plant physiology · 2010Article
- Why so repressed? Turning off transcription during plant growth and development.Current opinion in plant biology · 2009Review
- A feedback regulatory module formed by LITTLE ZIPPER and HD-ZIPIII genes.The Plant cell · 2007Article
- Characterization of the class IV homeodomain-Leucine Zipper gene family in Arabidopsis.Plant physiology · 2006Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Four groups of plant homeodomain proteins contain a dimerization motif closely linked to the homeodomain. We here show that two sunflower homeodomain proteins, Hahb-4 and HAHR1, which belong to the Hd-Zip I and GL2/Hd-Zip IV groups, respectively, show different binding preferences at a defined position of a pseudopalindromic DNA-binding site used as a target. HAHR1 shows a preference for the sequence 5'-CATT(A/T)AATG-3', rather than 5'-CAAT(A/T)ATTG-3', recognized by Hahb-4. To analyze the molecular basis of this behavior, we have constructed a set of mutants with exchanged residues (Phe-->Ile and Ile-->Phe) at position 47 of the homeodomain, together with chimeric proteins between HAHR1 and Hahb-4. The results obtained indicate that Phe47, but not Ile47, allows binding to 5'-CATT(A/T)AATG-3'. However, the preference for this sequence is determined, in addition, by amino acids located C-terminal to residue 53 of the HAHR1 homeodomain. A double mutant of Hahb-4 (Ile47-->Phe/Ala54-->Thr) shows the same binding behavior as HAHR1, suggesting that combinatorial interactions of amino acid residues at positions 47 and 54 of the homeodomain are involved in establishing the affinity and selectivity of plant dimeric homeodomain proteins with different DNA target sequences.
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