ArticleNucleic acids research2001
Nearest neighbour analysis of MCM protein complexes in Drosophila melanogaster.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Assembly of a homohexameric minichromosome maintenance complex is dependent on ATP and DNA.The Journal of biological chemistry · 2026Article
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Structure of human Cdc45 and implications for CMG helicase function.Nature communications · 2016Article
- A pipeline for determining protein-protein interactions and proximities in the cellular milieu.Molecular & cellular proteomics : MCP · 2014Article
- Aberrant expression of NEK2 and its clinical significance in non-small cell lung cancer.Oncology letters · 2014Article
- Examining Nek2 as a better proliferation marker in non-small cell lung cancer prognosis.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- The minichromosome maintenance replicative helicase.Cold Spring Harbor perspectives in biology · 2013Review
- Mechanisms for initiating cellular DNA replication.Annual review of biochemistry · 2013Review
- Functional analysis of Drosophila DNA polymerase ε p58 subunit.American journal of cancer research · 2013Article
- The structural basis for MCM2-7 helicase activation by GINS and Cdc45.Nature structural & molecular biology · 2011Article
- Article
- Activation of the MCM2-7 helicase by association with Cdc45 and GINS proteins.Molecular cell · 2010Article
- The Mcm complex: unwinding the mechanism of a replicative helicase.Microbiology and molecular biology reviews : MMBR · 2009Review
- Dynamic localization of the DNA replication proteins MCM5 and MCM7 in plants.Plant physiology · 2009Article
- Subunit organization of Mcm2-7 and the unequal role of active sites in ATP hydrolysis and viability.Molecular and cellular biology · 2008Article
- The human TPR protein TTC4 is a putative Hsp90 co-chaperone which interacts with CDC6 and shows alterations in transformed cells.PloS one · 2008Article
- Differential requirements for MCM proteins in DNA replication in Drosophila S2 cells.PloS one · 2007Article
- Cell cycle regulation of DNA replication.Annual review of genetics · 2007Review
- Minichromosome maintenance helicase activity is controlled by N- and C-terminal motifs and requires the ATPase domain helix-2 insert.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Eukaryotic MCM proteins: beyond replication initiation.Microbiology and molecular biology reviews : MMBR · 2004Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The MCM proteins are a group of six proteins whose action is vital for DNA replication in eukaryotes. It has been suggested that they constitute the replicative helicase, with a subset of the proteins forming the catalytic helicase (MCM4,6,7) while the others have a loading or control function. In this paper we show that all six MCM proteins are present in equivalent amounts in soluble extracts and on chromatin. We have also analysed soluble and chromatin-associated MCM protein complexes under different conditions. This suggests that all six MCM proteins are always found in a complex with each other, although the interaction between the individual MCM proteins is not equivalent as stringent salt conditions are able to break the intact complex into a number of stable subcomplexes. These data contribute to the ongoing debate about the nature of MCM complexes, supporting the hypothesis that they act as a heterohexamer rather than as a number of different subcomplexes. Finally, using protein-protein cross-linking we have shown that MCM2 interacts directly with MCM5 and MCM6; MCM5 with MCM3 and MCM2; and MCM6 with MCM2 and MCM4. This provides the first direct information about specific subunit contacts in the MCM complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.