ArticleNucleic acids research2001
A mRNA-based thermosensor controls expression of rhizobial heat shock genes.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 134 citations in OpenAlex.
- Mechanisms of thermoregulation inJournal of bacteriology · 2026Review
- Biosensor that Detects Stress Caused by Periplasmic Proteins.ACS synthetic biology · 2024Article
- An RNA thermometer in the chloroplast genome of Chlamydomonas facilitates temperature-controlled gene expression.Nucleic acids research · 2023Article
- Rock, scissors, paper: How RNA structure informs function.The Plant cell · 2023Article
- The effect of mutation in the stem of the MicroROSE thermometer on its thermosensing ability: insights from molecular dynamics simulation studies.RSC advances · 2022Article
- RNA structure mediated thermoregulation: What can we learn from plants?Frontiers in plant science · 2022Review
- Novel self-regulation strategy of a small heat shock protein for prodigious and rapid expression on demand.Current genetics · 2021Review
- Exploiting RNA thermometer-driven molecular bioprocess control as a concept for heterologous rhamnolipid production.Scientific reports · 2021Article
- A tight cold-inducible switch built by coupling thermosensitive transcriptional and proteolytic regulatory parts.Nucleic acids research · 2019Article
- Evaluating temperature-induced regulation of a ROSE-like RNA-thermometer for heterologous rhamnolipid production in Pseudomonas putida KT2440.AMB Express · 2019Article
- Bacterial riboswitches and RNA thermometers: Nature and contributions to pathogenesis.Non-coding RNA research · 2018Review
- RNA Thermometers in Bacterial Pathogens.Microbiology spectrum · 2018Review
- Auxotrophy to Xeno-DNA: an exploration of combinatorial mechanisms for a high-fidelity biosafety system for synthetic biology applications.Journal of biological engineering · 2018Review
- Exploring the modular nature of riboswitches and RNA thermometers.Nucleic acids research · 2016Article
- RNAiFold2T: Constraint Programming design of thermo-IRES switches.Bioinformatics (Oxford, England) · 2016Article
- Stressing out over long noncoding RNA.Biochimica et biophysica acta · 2016Review
- De novo design of heat-repressible RNA thermosensors in E. coli.Nucleic acids research · 2015Article
- Mechanistic insights into temperature-dependent regulation of the simple cyanobacterial hsp17 RNA thermometer at base-pair resolution.Nucleic acids research · 2015Article
- Secondary structural entropy in RNA switch (Riboswitch) identification.BMC bioinformatics · 2015Article
- Site-specific fluorescence dynamics in an RNA 'thermometer' reveals the role of ribosome binding in its temperature-sensitive switch function.Nucleic acids research · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of several heat shock operons, mainly coding for small heat shock proteins, is under the control of ROSE (repression of heat shock gene expression) in various rhizobial species. This negatively cis-acting element confers temperature control by preventing expression at physiological temperatures. We provide evidence that ROSE-mediated regulation occurs at the post-transcriptional level. A detailed mutational analysis of ROSE(1)-hspA translationally fused to lacZ revealed that its highly conserved 3'-half is required for repression at normal temperatures (30 degrees C). The mRNA in this region is predicted to form an extended secondary structure that looks very similar in all 15 known ROSE elements. Nucleotides involved in base pairing are strongly conserved, whereas nucleotides in loop regions are more divergent. Base substitutions leading to derepression of the lacZ fusion at 30 degrees C exclusively resided in potential stem structures. Optimised base pairing by elimination of a bulged residue and by introduction of complementary nucleotides in internal loops resulted in ROSE elements that were tightly repressed not only at normal but also at heat shock temperatures. We propose a model in which the temperature-regulated secondary structure of ROSE mRNA influences heat shock gene expression by controlling ribosome access to the ribosome-binding site.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.