ArticleInvestigative ophthalmology & visual science2001
Gene transfer mediated by recombinant baculovirus into mouse eye.
Article in Investigative ophthalmology & visual science, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 51 citations in OpenAlex.
- A High-Multiplicity Baculovirus Method Enables Efficient Gene Delivery to Diverse Mammalian Cells In Vitro and to Multiple Organs In Vivo.International journal of molecular sciences · 2025Article
- Overexpression of GDNF in Spinal Cord Attenuates Morphine Analgesic Tolerance in Rats with Bone Cancer Pain.Brain sciences · 2022Article
- Viral-Vector-Delivered Anti-Angiogenic Therapies to the Eye.Pharmaceutics · 2021Review
- Spatial control of in vivo CRISPR-Cas9 genome editing via nanomagnets.Nature biomedical engineering · 2019Article
- Highly efficient transduction of primary adult CNS and PNS neurons.Scientific reports · 2016Article
- In Vitro Cell Models for Ophthalmic Drug Development Applications.BioResearch open access · 2016Review
- Review
- Innate immune response induced by baculovirus attenuates transgene expression in mammalian cells.Journal of virology · 2014Article
- Baculovirus: an insect-derived vector for diverse gene transfer applications.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Review
- Screening of complement inhibitors: shielded baculoviruses increase the safety and efficacy of gene delivery.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Interstitial tissue-specific gene expression in mouse testis by intra-tunica albuguineal injection of recombinant baculovirus.Asian journal of andrology · 2009Article
- Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells.PloS one · 2009Article
- In vivo transcriptional targeting into the retinal vasculature using recombinant baculovirus carrying the human flt-1 promoter.Virology journal · 2007Article
- SPECT/CT imaging of baculovirus biodistribution in rat.Gene therapy · 2007Article
- Lentiviral mediated gene delivery to the anterior chamber of rodent eyes.Molecular vision · 2005Article
- Neural retina limits the nonviral gene transfer to retinal pigment epithelium in an in vitro bovine eye model.The AAPS journal · 2004Article
- Essential role of Ca2+-binding protein 4, a Cav1.4 channel regulator, in photoreceptor synaptic function.Nature neuroscience · 2004Article
- In vitro and in vivo gene delivery by recombinant baculoviruses.Journal of virology · 2003Article
- Immune response following intraocular delivery of recombinant viral vectors.Gene therapy · 2003Review
- Baculoviruses as Vectors for Gene Therapy against Human Prostate Cancer.Journal of biomedicine & biotechnology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo determine the efficiency of baculoviruses (BVs) to transfer recombinant genes in vivo into murine ocular tissues.
methodsRecombinant (r)BVs carrying fluorescent protein (FP) cDNA under the control of cytomegalovirus (CMV) immediate early promoter were constructed. Initially, cultured HEK293 and ARPE19 cells were infected with these rBVs and analyzed for efficiency and stability of transgene expression. The rBV-CMV green (G)FP was also injected into the intravitreal and subretinal space of mouse eye. Mice were periodically analyzed to determine the efficiency and stability of expression by histologic examination under fluorescence microscopy. The effect of rBV-CMV-GFP on the physiology of the retina was analyzed by electroretinography.
resultscDNAs encoding fluorescent proteins were efficiently transduced in HEK293 and ARPE19 cells in vitro. GFP expression in vivo was observed exclusively in retinal pigment epithelial (RPE) cells after subretinal injections. Intravitreal injections of rBV resulted in GFP expression in the corneal endothelium, lens, RPE, and retina. GFP expression was observed for up to 14 days after injection. The infiltration of macrophages, observed 2 days after injection in the area of GFP transduction, had dissipated by day 8 after injection. No alteration in ERG responses was observed 6 weeks after injection of rBV-CMV-GFP.
conclusionsBV efficiently transduces cultured RPE cells and many cell types in vivo in the eye, including endothelial, epithelial, and neuronal cells. BV may be a useful vector for transferring genes in cultured cells and in vivo into ocular tissue.
Indexed as
Identifiers
11726636PMC1436034W2145028780What OpenQuestion holds
Registered trials
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