ArticleInvestigative ophthalmology & visual science2001
Atrophy of Müller glia and photoreceptor cells in chick retina misexpressing cNSCL2.
Article in Investigative ophthalmology & visual science, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- Arcuate Nucleus Overexpression of NHLH2 Reduces Body Mass and Attenuates Obesity-Associated Anxiety/Depression-like Behavior.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Mechanism of the specific neuronal toxicity of a type I ribosome-inactivating protein, trichosanthin.Neurotoxicity research · 2010Article
- Neurogenin1 effectively reprograms cultured chick retinal pigment epithelial cells to differentiate toward photoreceptors.The Journal of comparative neurology · 2010Article
- Pro-photoreceptor activity of chick neurogenin1.Investigative ophthalmology & visual science · 2009Article
- Reprogramming retinal pigment epithelium to differentiate toward retinal neurons with Sox2.Stem cells (Dayton, Ohio) · 2009Article
- Neurogenin3 promotes early retinal neurogenesis.Molecular and cellular neurosciences · 2009Article
- bHLH genes and retinal cell fate specification.Molecular neurobiology · 2005Review
- A role of ath5 in inducing neuroD and the photoreceptor pathway.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Requirement of neuroD for photoreceptor formation in the chick retina.Investigative ophthalmology & visual science · 2004Article
- Proneural gene ash1 promotes amacrine cell production in the chick retina.Developmental neurobiologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo investigate whether and how the basic helix-loop-helix (bHLH) gene cNSCL2 is involved in retinal development.
methodscNSCL2, the chick homologue of human NSCL2, was isolated and sequenced. In situ hybridization was used to examine its spatial and temporal expression pattern in the retina. Replication-competent retrovirus RCAS was used to drive cNSCL2 misexpression in the developing chick retina, and the effect of the misexpression was analyzed.
resultsExpression of cNSCL2 in the retina was restricted. Its mRNA was detected in amacrine and horizontal cells, but not in photoreceptor, bipolar, or ganglion cells. Retroviral-driven misexpression of cNSCL2 in the developing chick retina resulted in missing photoreceptor cells and gross deficits in the outer nuclear layer (ONL). These deficits were probably not because of decreased photoreceptor production, in that the ONL appeared normal in early developmental stages. TUNEL+ cells were detected in the ONL, indicating that photoreceptor cells underwent apoptosis in retinas misexpressing cNSCL2. Müller glial cells were far fewer in the experimental retina than in the control, indicating that cNSCL2 also caused Müller glia atrophy. The onset of Müller glia disappearance preceded that of photoreceptor degeneration.
conclusionsExpression of cNSCL2 in the chick retina was restricted to amacrine and horizontal cells. Misexpression of cNSCL2 caused severe retinal degeneration, and photoreceptor cells and Müller glia were particularly affected.
Indexed as
Identifiers
11726609PMC1986830W1591542759What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.