ArticleThe EMBO journal2001
Release of U18 snoRNA from its host intron requires interaction of Nop1p with the Rnt1p endonuclease.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed.
- Article
- Small Nucleolar RNAs (snoRNAs) in Cancer: From Biogenesis to Clinical Potential.OncoTargets and therapy · 2026Review
- Subverting the Canon: Novel Cancer-Promoting Functions and Mechanisms for snoRNAs.International journal of molecular sciences · 2024Review
- RNA helicase-mediated regulation of snoRNP dynamics on pre-ribosomes and rRNA 2'-O-methylation.Nucleic acids research · 2021Article
- Biogenesis of RNase P RNA from an intron requires co-assembly with cognate protein subunits.Nucleic acids research · 2019Article
- Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage.Nature communications · 2018Article
- Loss of the Yeast SR Protein Npl3 Alters Gene Expression Due to Transcription Readthrough.PLoS genetics · 2015Article
- Global analysis of RNA cleavage by 5'-hydroxyl RNA sequencing.Nucleic acids research · 2015Article
- Small nucleolar RNAs functioning and potential roles in cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Review
- Reporter mRNAs cleaved by Rnt1p are exported and degraded in the cytoplasm.Nucleic acids research · 2011Article
- The spatial-functional coupling of box C/D and C'/D' RNPs is an evolutionarily conserved feature of the eukaryotic box C/D snoRNP nucleotide modification complex.Molecular and cellular biology · 2011Article
- Processing of a dicistronic tRNA-snoRNA precursor: combined analysis in vitro and in vivo reveals alternate pathways and coupling to assembly of snoRNP.Plant physiology · 2009Article
- Genome-wide search for yeast RNase P substrates reveals role in maturation of intron-encoded box C/D small nucleolar RNAs.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Characterization of a ribonuclease III-like protein required for cleavage of the pre-rRNA in the 3'ETS in Arabidopsis.Nucleic acids research · 2008Article
- The position of yeast snoRNA-coding regions within host introns is essential for their biosynthesis and for efficient splicing of the host pre-mRNA.RNA (New York, N.Y.) · 2007Article
- The structure of the endoribonuclease XendoU: From small nucleolar RNA processing to severe acute respiratory syndrome coronavirus replication.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- The cotranscriptional assembly of snoRNPs controls the biosynthesis of H/ACA snoRNAs in Saccharomyces cerevisiae.Molecular and cellular biology · 2005Article
- Viral class 1 RNase III involved in suppression of RNA silencing.Journal of virology · 2005Article
- The RNA catabolic enzymes Rex4p, Rnt1p, and Dbr1p show genetic interaction with trans-acting factors involved in processing of ITS1 in Saccharomyces cerevisiae pre-rRNA.RNA (New York, N.Y.) · 2004Article
- Coupling between snoRNP assembly and 3' processing controls box C/D snoRNA biosynthesis in yeast.The EMBO journal · 2004Article
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4 authors.
Funding
Abstract
An external stem, essential for the release of small nucleolar RNAs (snoRNAs) from their pre-mRNAs, flanks the majority of yeast intron-encoded snoRNAs. Even if this stem is not a canonical Rnt1p substrate, several experiments have indicated that the Rnt1p endonuclease is required for snoRNA processing. To identify the factors necessary for processing of intron-encoded snoRNAs, we have raised in vitro extracts able to reproduce such activity. We found that snoRNP factors are associated with the snoRNA- coding region throughout all the processing steps, and that mutants unable to assemble snoRNPs have a processing-deficient phenotype. Specific depletion of Nop1p completely prevents U18 snoRNA synthesis, but does not affect processing of a dicistronic snoRNA-coding unit that has a canonical Rnt1p site. Correct cleavage of intron-encoded U18 and snR38 snoRNAs can be reproduced in vitro by incubating together purified Nop1p and Rnt1p. Pull-down experiments showed that the two proteins interact physically. These data indicate that cleavage of U18, snR38 and possibly other intron-encoded snoRNAs is a regulated process, since the stem is cleaved by the Rnt1p endonuclease only when snoRNP assembly has occurred.
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