ArticleThe EMBO journal2001
Fission yeast Rad50 stimulates sister chromatid recombination and links cohesion with repair.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 120 citations in OpenAlex.
- The Molecular Mechanisms and Therapeutic Prospects of Alternative Lengthening of Telomeres (ALT).Cancers · 2023Review
- In silico analysis of a novel pathogenic variant c.7G > A in C14orf39 gene identified by WES in a Pakistani family with azoospermia.Molecular genetics and genomics : MGG · 2022Article
- Mre11 exonuclease activity removes the chain-terminating nucleoside analog gemcitabine from the nascent strand during DNA replication.Science advances · 2020Article
- Rad50 zinc hook functions as a constitutive dimerization module interchangeable with SMC hinge.Nature communications · 2020Article
- Review
- ATRX affects the repair of telomeric DSBs by promoting cohesion and a DAXX-dependent activity.PLoS biology · 2020Article
- Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast.Molecular biology of the cell · 2019Article
- Histone deposition promotes recombination-dependent replication at arrested forks.PLoS genetics · 2019Article
- XPG-related nucleases are hierarchically recruited for double-stranded rDNA break resection.The Journal of biological chemistry · 2019Article
- Review
- Pathways and Mechanisms that Prevent Genome Instability inGenetics · 2017Review
- Article
- C14ORF39/SIX6OS1 is a constituent of the synaptonemal complex and is essential for mouse fertility.Nature communications · 2016Article
- Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.G3 (Bethesda, Md.) · 2016Article
- The distribution of α-kleisin during meiosis in the holocentromeric plant Luzula elegans.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016Article
- Ultrastructural changes associated with the induction of premature chromosome condensation in Vicia faba root meristem cells.Plant cell reports · 2014Article
- The Rad50 hook domain regulates DNA damage signaling and tumorigenesis.Genes & development · 2014Article
- Competing roles of DNA end resection and non-homologous end joining functions in the repair of replication-born double-strand breaks by sister-chromatid recombination.Nucleic acids research · 2013Article
- The cohesin subunit RAD21L functions in meiotic synapsis and exhibits sexual dimorphism in fertility.The EMBO journal · 2011Article
- The MRE11 complex: starting from the ends.Nature reviews. Molecular cell biology · 2011Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To study the role of Rad50 in the DNA damage response, we cloned and deleted the Schizosaccharomyces pombe RAD50 homologue. The deletion is sensitive to a range of DNA-damaging agents and shows dynamic epistatic interactions with other recombination-repair genes. We show that Rad50 is necessary for recombinational repair of the DNA lesion at the mating-type locus and that rad50Delta shows slow DNA replication. We also find that Rad50 is not required for slowing down S phase in response to hydroxy urea or methyl methanesulfonate (MMS) treatment. Interestingly, in rad50Delta cells, the recombination frequency between two homologous chromosomes is increased at the expense of sister chromatid recombination. We propose that Rad50, an SMC-like protein, promotes the use of the sister chromatid as the template for homologous recombinational repair. In support of this, we found that Rad50 functions in the same pathway for the repair of MMS-induced damage as Rad21, the homologue of the Saccharomyces cerevisiae Scc1 cohesin protein. We speculate that Rad50 interacts with the cohesin complex during S phase to assist repair and possibly re-initiation of replication after replication fork collapse.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.