ArticleThe EMBO journal2001
Crystal structure of the CENP-B protein-DNA complex: the DNA-binding domains of CENP-B induce kinks in the CENP-B box DNA.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 120 citations in OpenAlex.
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- Conservation of dichromatin organization along regional centromeres.Cell genomics · 2025Article
- CENP-A/CENP-B uncoupling in the evolutionary reshuffling of centromeres in equids.Genome biology · 2025Article
- Vertebrate centromere architecture: from chromatin threads to functional structures.Chromosoma · 2024Review
- Dan forms condensates in neuroblasts and regulates nuclear architecture and progenitor competence in vivo.Nature communications · 2024Article
- The structure, function, and evolution of plant centromeres.Genome research · 2024Review
- DNA satellite and chromatin organization at mouse centromeres and pericentromeres.Genome biology · 2024Article
- [Clinical and immunological features of primary Sjögren's syndrome patients with positive anti-centromere protein B antibody].Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2023Article
- CENP-A and CENP-B collaborate to create an open centromeric chromatin state.Nature communications · 2023Article
- Centromere innovations within a mouse species.Science advances · 2023Article
- ERCC6L2 mitigates replication stress and promotes centromere stability.Cell reports · 2023Article
- IS21 family transposase cleaved donor complex traps two right-handed superhelical crossings.Nature communications · 2023Article
- Enrichment of Non-B-Form DNA at D. melanogaster Centromeres.Genome biology and evolution · 2022Article
- The genetics and epigenetics of satellite centromeres.Genome research · 2022Article
- Structural studies of functional nucleosome complexes with transacting factors.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2022Review
- CENP-A: A Histone H3 Variant with Key Roles in Centromere Architecture in Healthy and Diseased States.Results and problems in cell differentiation · 2022Article
- Diverse mechanisms of centromere specification.Current biology : CB · 2021Review
- Structural and dynamic mechanisms of CBF3-guided centromeric nucleosome formation.Nature communications · 2021Article
- A comprehensive transcription factor and DNA-binding motif resource for the construction of gene regulatory networks inComputational and structural biotechnology journal · 2021Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human centromere protein B (CENP-B), one of the centromere components, specifically binds a 17 bp sequence (the CENP-B box), which appears in every other alpha-satellite repeat. In the present study, the crystal structure of the complex of the DNA-binding region (129 residues) of CENP-B and the CENP-B box DNA has been determined at 2.5 A resolution. The DNA-binding region forms two helix-turn-helix domains, which are bound to adjacent major grooves of the DNA. The DNA is kinked at the two recognition helix contact sites, and the DNA region between the kinks is straight. Among the major groove protein-bound DNAs, this 'kink-straight-kink' bend contrasts with ordinary 'round bends' (gradual bending between two protein contact sites). The larger kink (43 degrees ) is induced by a novel mechanism, 'phosphate bridging by an arginine-rich helix': the recognition helix with an arginine cluster is inserted perpendicularly into the major groove and bridges the groove through direct interactions with the phosphate groups. The overall bending angle is 59 degrees, which may be important for the centromere-specific chromatin structure.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.