ArticleProceedings of the National Academy of Sciences of the United States of America2001
Signaling at the inhibitory natural killer cell immune synapse regulates lipid raft polarization but not class I MHC clustering.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 115 citations in OpenAlex.
- Immunological synapse: structures, molecular mechanisms and therapeutic implications in disease.Signal transduction and targeted therapy · 2025Review
- KIR signaling is regulated by electrostatic interaction of its cytosolic tail with the plasma membrane despite being neutral polyampholyte.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Assessment of membrane lipid state at the natural killer cell immunological synapse.Methods in cell biology · 2023Article
- Evasion of NK cell immune surveillanceFrontiers in immunology · 2022Article
- Degranulation enhances presynaptic membrane packing, which protects NK cells from perforin-mediated autolysis.PLoS biology · 2021Article
- The Role of the Cytoskeleton in Regulating the Natural Killer Cell Immune Response in Health and Disease: From Signaling Dynamics to Function.Frontiers in cell and developmental biology · 2021Review
- Myosin 1g and 1f: A Prospective Analysis in NK Cell Functions.Frontiers in immunology · 2021Review
- Mechanosensation and Mechanotransduction in Natural Killer Cells.Frontiers in immunology · 2021Review
- GMP-Compliant Universal Antigen Presenting Cells (uAPC) Promote the Metabolic Fitness and Antitumor Activity of Armored Cord Blood CAR-NK Cells.Frontiers in immunology · 2021Article
- Considerations of Antibody Geometric Constraints on NK Cell Antibody Dependent Cellular Cytotoxicity.Frontiers in immunology · 2020Review
- Review
- T cell immunoengineering with advanced biomaterials.Integrative biology : quantitative biosciences from nano to macro · 2017Review
- CD160 is essential for NK-mediated IFN-γ production.The Journal of experimental medicine · 2015Article
- L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration.Journal of leukocyte biology · 2014Article
- Article
- Understanding natural killer cell regulation by mathematical approaches.Frontiers in immunology · 2012Article
- Activation or tolerance of natural killer cells is modulated by ligand quality in a nonmonotonic manner.Biophysical journal · 2010Article
- Quantitative measurement of F-actin accumulation at the NK cell immunological synapse.Journal of immunological methods · 2010Article
- Significant functional heterogeneity among KIR2DL1 alleles and a pivotal role of arginine 245.Blood · 2009Article
- Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Natural killer (NK) cell cytotoxicity is determined by a balance of positive and negative signals. Negative signals are transmitted by NK inhibitory receptors (killer immunoglobulin-like receptors, KIR) at the site of membrane apposition between an NK cell and a target cell, where inhibitory receptors become clustered with class I MHC ligands in an organized structure known as an inhibitory NK immune synapse. Immune synapse formation in NK cells is poorly understood. Because signaling by NK inhibitory receptors could be involved in this process, the human NK tumor line YTS was transfected with signal-competent and signal-incompetent KIR2DL1. The latter were generated by truncating the KIR2DL1 cytoplasmic tail or by introducing mutations in the immunoreceptor tyrosine-based inhibition motifs. The KIR2DL1 mutants retained their ability to cluster class I MHC ligands on NK cell interaction with appropriate target cells. Therefore, receptor-ligand clustering at the inhibitory NK immune synapse occurs independently of KIR2DL1 signal transduction. However, parallel examination of NK cell membrane lipid rafts revealed that KIR2DL1 signaling is critical for blocking lipid raft polarization and NK cell cytotoxicity. Moreover, raft polarization was inhibited by reagents that disrupt microtubules and actin filaments, whereas synapse formation was not. Thus, NK lipid raft polarization and inhibitory NK immune synapse formation occur by fundamentally distinct mechanisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.