ArticleJournal of clinical microbiology2001
European proficiency testing program for molecular detection and quantitation of hepatitis B virus DNA.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Downregulation of Toll-Like Receptor Gene Expression Among Hepatitis B Virus-Positive Human Patients.Cureus · 2025Article
- Article
- Ring test evaluation of the detection of influenza A virus in swine oral fluids by real-time reverse-transcription polymerase chain reaction and virus isolation.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2016Article
- Development of magnetic capture hybridization and quantitative polymerase chain reaction for hepatitis B virus covalently closed circular DNA.Hepatitis monthly · 2015Article
- Multicenter external quality assessment program for PCR detection of Mycobacterium ulcerans in clinical and environmental specimens.PloS one · 2014Article
- Global proficiency study of human papillomavirus genotyping.Journal of clinical microbiology · 2010Article
- Multicenter external quality assessment of molecular methods for detection of human herpesvirus 6.Journal of clinical microbiology · 2010Article
- TREAT Asia Quality Assessment Scheme (TAQAS) to standardize the outcome of HIV genotypic resistance testing in a group of Asian laboratories.Journal of virological methods · 2009Article
- Detection and quantification of hepatitis B virus DNA by SYBR green real-time polymerase chain reaction.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2007Article
- Follow-up on diagnostic proficiency of laboratories equipped to perform orthopoxvirus detection and quantification by PCR: the second international external quality assurance study.Journal of clinical microbiology · 2006Article
- Two quality control exercises involving nucleic acid amplification methods for detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae and carried out 2 years apart (in 2002 and 2004).Journal of clinical microbiology · 2006Article
- Results of the first World Health Organization international collaborative study of detection of human papillomavirus DNA.Journal of clinical microbiology · 2006Article
- External quality assessment for molecular detection of Bordetella pertussis in European laboratories.Journal of clinical microbiology · 2005Article
- Assessment of the target-capture PCR hepatitis B virus (HBV) DNA quantitative assay and comparison with commercial HBV DNA quantitative assays.Journal of clinical microbiology · 2004Article
- Fully automated, internally controlled quantification of hepatitis B Virus DNA by real-time PCR by use of the MagNA Pure LC and LightCycler instruments.Journal of clinical microbiology · 2004Article
- Molecular and diagnostic clinical virology in real time.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2004Review
- Reliability of nucleic acid amplification methods for detection of Chlamydia trachomatis in urine: results of the first international collaborative quality control study among 96 laboratories.Journal of clinical microbiology · 2003Article
- Clinical virology in real time.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2002Review
- External quality assessment program for qualitative and quantitative detection of hepatitis C virus RNA in diagnostic virology.Journal of clinical microbiology · 2002Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
External quality control of hepatitis B virus (HBV) DNA detection remains an important issue. This study reports and compares the results obtained from two different proficiency panels for both the qualitative and quantitative assessment of HBV DNA. The panels were designed by the European Union Quality Control Concerted Action, prepared by Boston Biomedica, Inc., and distributed in May 1999 (panel 1) and February 2000 (panel 2). Each contained two negative samples and six positive samples with 10(3) to 10(7) copies/ml (panel 1) or 10(3) to 2 x 10(6) copies of HBV DNA per ml (panel 2). For panel 1, 42 laboratories submitted 20 qualitative (all in-house PCRs) and 37 quantitative (87% commercial assays) data sets. For panel 2, 51 laboratories submitted 25 qualitative (all in-house PCRs) and 47 quantitative (94% commercial assays) data sets. Five data sets (8.8%) in panel 1 and two data sets (2.8%) in panel 2 contained totals of six and two false-positives, respectively, corresponding to false-positive result rates of 5.3% for panel 1 and 1.4% for panel 2. The false-negative result rates of 10.5% for panel 1 and 17.4% for panel 2 were dependent on the detection levels of the assays employed as well as panel composition. In the qualitative analysis of all data sets, 47.4% (panel 1) and 51.4% (panel 2) had all samples correct. An adequate or better score (all correct or only the weak-positive sample missed) was obtained with 77.2% of the panel 1 samples and 68.1% of the panel 2 samples. In the quantitative analysis, 57.1% (panel 1) and 42.6% (panel 2) of the data sets achieved an adequate or better score (positive results within the acceptable range of the geometric mean +/- 0.5 log(10) of all positive results). These results demonstrate that while the qualitative performance of HBV detection has considerably improved compared to that of a previously published HBV proficiency study, the detection levels of many commercial quantitative assays are still too high to allow adequate quantitation of all relevant clinical samples.
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