ArticleJournal of clinical microbiology2001
Characterization of capsid genes, expressed in the baculovirus system, of three new genetically distinct strains of "Norwalk-like viruses".
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 62 citations in OpenAlex.
- Intestinal Norovirus Binding Patterns in Nonsecretor Individuals.Journal of virology · 2022Article
- Profiling of humoral immune responses to norovirus in children across Europe.Scientific reports · 2022Article
- Epidemiological Impact of GII.17 Human Noroviruses Associated With Attachment to Enterocytes.Frontiers in microbiology · 2022Article
- Free Chlorine and Peroxynitrite Alter the Capsid Structure of Human Norovirus GII.4 and Its Capacity to Bind Histo-Blood Group Antigens.Frontiers in microbiology · 2021Article
- A Luciferase Immunoprecipitation System (LIPS) assay for profiling human norovirus antibodies.Journal of virological methods · 2017Article
- Characterization of virus-like particles derived from a GII.3 norovirus strain distantly related with current dominating strains.Virus genes · 2016Article
- Bovine noroviruses: A missing component of calf diarrhoea diagnosis.Veterinary journal (London, England : 1997) · 2016Review
- Evidence for human norovirus infection of dogs in the United kingdom.Journal of clinical microbiology · 2015Article
- Absolute Humidity Influences the Seasonal Persistence and Infectivity of Human Norovirus.Applied and environmental microbiology · 2014Article
- Particle conformation regulates antibody access to a conserved GII.4 norovirus blockade epitope.Journal of virology · 2014Article
- Identification and characterization of antibody-binding epitopes on the norovirus GII.3 capsid.Journal of virology · 2014Article
- Serological evidence for multiple strains of canine norovirus in the UK dog population.PloS one · 2013Article
- Bioluminescent enzyme immunoassay for the detection of norovirus capsid antigen.Clinical and vaccine immunology : CVI · 2012Article
- Serosurvey of veterinary conference participants for evidence of zoonotic exposure to canine norovirus - study protocol.Virology journal · 2012Article
- Qualitative and quantitative analysis of the binding of GII.4 norovirus variants onto human blood group antigens.Journal of virology · 2011Article
- Immunogenicity and safety of virus-like particle of the porcine encephalomyocarditis virus in pig.Virology journal · 2011Article
- A comparison of methods for purification and concentration of norovirus GII-4 capsid virus-like particles.Archives of virology · 2010Article
- Expression and self-assembly of virus-like particles from two genotypes of marine vesiviruses and development of an ELISA for the detection of antibodies.Veterinary microbiology · 2010Article
- Heterotypic humoral and cellular immune responses following Norwalk virus infection.Journal of virology · 2010Article
- Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs.Veterinary microbiology · 2009Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
"Norwalk-like viruses" (NLVs), members of a newly defined genus of the family Caliciviridae, are the most common agents of outbreaks of gastroenteritis in the United States. Two features of NLVs have hindered the development of simple methods for detection and determination of serotype: their genetic diversity and their inability to grow in cell culture. To assess the immune responses of patients involved in outbreaks of gastroenteritis resulting from infection with NLVs, we previously used recombinant-expressed capsid antigens representing four different genetic clusters, but this panel proved insufficient for detection of an immune response in many patients. To extend and further refine this panel, we expressed in baculovirus the capsid genes of three additional genetically distinct viruses, Burwash Landing virus (BLV), White River virus (WRV), and Florida virus. All three expressed proteins assembled into virus-like particles (VLPs) that contained a full-length 64-kDa protein, but both the BLV and WRV VLPs also contained a 58-kDa protein that resulted from deletion of 39 amino acids at the amino terminus. The purified VLPs were used to measure the immune responses in 403 patients involved in 37 outbreaks of acute gastroenteritis. A majority of patients demonstrated a fourfold rise in the titer of immunoglobulin G to the antigen homologous to the outbreak strain, but most seroconverted in response to other genetically distinct antigens as well, suggesting no clear pattern of type-specific immune response. Further study of the antigenicity of the NLVs by use of VLPs should allow us to design new detection systems with either broader reactivity or better specificity and to define the optimum panel of antigens required for routine screening of patient sera.
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