ArticleJournal of clinical microbiology2001
Quantification of proviral load of human immunodeficiency virus type 2 subtypes A and B using real-time PCR.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Point-of-care viral load tests to detect high HIV viral load in people living with HIV/AIDS attending health facilities.The Cochrane database of systematic reviews · 2022Pooled it
- The HIV-2 OGH double reporter virus shows that HIV-2 is less cytotoxic and less sensitive to reactivation from latency than HIV-1 in cell culture.Journal of virus eradication · 2023Article
- Article
- Genetic Variability of Long Terminal Repeat Region between HIV-2 Groups Impacts Transcriptional Activity.Journal of virology · 2020Article
- Quantification of HIV-2 DNA in Whole Blood.Bio-protocol · 2019Article
- New Highly Sensitive Real-Time PCR Assay for HIV-2 Group A and Group B DNA Quantification.Journal of clinical microbiology · 2017Article
- Modern-day SIV viral diversity generated by extensive recombination and cross-species transmission.PLoS pathogens · 2017Article
- Clinical validation of a novel diagnostic HIV-2 total nucleic acid qualitative assay using the Abbott m2000 platform: Implications for complementary HIV-2 nucleic acid testing for the CDC 4th generation HIV diagnostic testing algorithm.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2017Article
- The Molecular Characterization of Intestinal Explant HIV Infection Using Polymerase Chain Reaction-Based Techniques.AIDS research and human retroviruses · 2015Article
- Characterization of small ruminant lentivirus A4 subtype isolates and assessment of their pathogenic potential in naturally infected goats.Virology journal · 2014Article
- Re-testing and misclassification of HIV-2 and HIV-1&2 dually reactive patients among the HIV-2 cohort of the West African Database to evaluate AIDS collaboration.Journal of the International AIDS Society · 2014Article
- High level of susceptibility to human TRIM5α conferred by HIV-2 capsid sequences.Retrovirology · 2013Article
- Origins of HIV and the AIDS pandemic.Cold Spring Harbor perspectives in medicine · 2011Review
- Downregulation of the T-cell receptor by human immunodeficiency virus type 2 Nef does not protect against disease progression.Journal of virology · 2009Article
- Differences in proviral DNA load between HIV-1-infected and HIV-2-infected patients.AIDS (London, England) · 2008Article
- Real-time PCR quantification of genital shedding of herpes simplex virus (HSV) and human immunodeficiency virus (HIV) in women coinfected with HSV and HIV.Journal of clinical microbiology · 2006Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability.Nucleic acids research · 2005Article
- Rapid discrimination between human immunodeficiency virus type 2 groups A and B by real-time PCR.Journal of clinical microbiology · 2004Article
- Field evaluation of a rapid human immunodeficiency virus (HIV) serial serologic testing algorithm for diagnosis and differentiation of HIV type 1 (HIV-1), HIV-2, and dual HIV-1-HIV-2 infections in West African pregnant women.Journal of clinical microbiology · 2004Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed and evaluated a new method to quantify human immunodeficiency virus type 2 (HIV-2) proviral DNA based on LightCycler real-time PCR. The assay has a detection limit of 5 copies/10(5) peripheral blood mononuclear cells (PBMC) and is insensitive to HIV-2 strain variability: HIV-2 subtypes A and B are both recognized and quantified. The intra- and interassay coefficients of variation range from 16 to 40% for high provirus concentrations (5 x 10(5) copies) and from 41 to 39% for low concentrations (5 copies). We used this method to compare the proviral DNA load and viral RNA load in plasma with clinical and immunological status for 29 patients infected by HIV-2 (subtype A in 17 and subtype B in 12). The proviral load (median, 201 copies/10(5) PBMC) was similar to that reported for HIV-1 infection. The median proviral loads did not correlate with the CD4(+) cell count categories and were as follows for CD4(+) cell counts of >400, 200 to 400, and <200 cells/mm(3), respectively: 121 copies/10(5) PBMC (n = 8; range, <5 to 712 copies/10(5) PBMC); 114 copies/10(5) PBMC (n = 9; range, <5 to 1,907 copies/10(5) PBMC); and 285 copies/10(5) PBMC (n = 12; range, 53 to 2,524 copies/10(5) PBMC). Proviral load did not correlate with plasma HIV-2 RNA positivity. As HIV-2 is considered to replicate less efficiently than HIV-1, these high proviral loads might be explained by the proliferation of infected cells.
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